Identification and Characterization of a New Staphylococcal Enterotoxin-Related Putative Toxin Encoded by Two Kinds of Plasmids

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  • Katsuhiko Omoe
    Department of Veterinary Microbiology, Faculty of Agriculture, Iwate University, Morioka, Iwate 020-8550
  • Dong-Liang Hu
    Department of Bacteriology, Hirosaki University School of Medicine, Zaifu-cho, Hirosaki 036-8562
  • Hiromi Takahashi-Omoe
    Department of Technical Support and Development, National Institute of Radiological Sciences, Inage-ku, Chiba-shi 263-8555, Japan
  • Akio Nakane
    Department of Bacteriology, Hirosaki University School of Medicine, Zaifu-cho, Hirosaki 036-8562
  • Kunihiro Shinagawa
    Department of Veterinary Microbiology, Faculty of Agriculture, Iwate University, Morioka, Iwate 020-8550

抄録

<jats:title>ABSTRACT</jats:title> <jats:p> We identified and characterized a novel staphylococcal enterotoxin-like putative toxin, which is named SER. Nucleotide sequencing analysis of the <jats:italic>ser</jats:italic> gene revealed that <jats:italic>ser</jats:italic> was most closely related to the <jats:italic>seg</jats:italic> gene. The <jats:italic>ser</jats:italic> gene product, SER, was successfully expressed as a recombinant protein in an <jats:italic>Escherichia coli</jats:italic> expression system, and recombinant SER (rSER) showed significant T-cell stimulation activity. The SER production in <jats:italic>ser</jats:italic> -harboring <jats:italic>Staphylococcus aureus</jats:italic> strains was confirmed by Western blot analysis using anti-rSER antibody. Moreover, <jats:italic>ser</jats:italic> was seen to be encoded by at least two types of plasmids. In particular, one kind of plasmid encoding the <jats:italic>ser</jats:italic> gene has been known as a <jats:italic>sed</jats:italic> - and <jats:italic>sej</jats:italic> -carrying pIB485-related plasmid. </jats:p>

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