ITIH4 (Inter-Alpha-Trypsin Inhibitor Heavy Chain 4) Is a New Acute-Phase Protein Isolated from Cattle during Experimental Infection

• M. Piñeiro

  Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Ciencias, Universidad de Zaragoza, 50009 Zaragoza, Spain

• M. Andrés

  Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Ciencias, Universidad de Zaragoza, 50009 Zaragoza, Spain

• M. Iturralde

  Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Ciencias, Universidad de Zaragoza, 50009 Zaragoza, Spain

• S. Carmona

  Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Ciencias, Universidad de Zaragoza, 50009 Zaragoza, Spain

• J. Hirvonen

  Department of Clinical Veterinary Sciences, Faculty of Veterinary Medicine, University of Helsinki, Saari Unit, FIN-04920 Saarentaus, Finland

• S. Pyörälä

  Department of Clinical Veterinary Sciences, Faculty of Veterinary Medicine, University of Helsinki, Saari Unit, FIN-04920 Saarentaus, Finland

• P. M. H. Heegaard

  Department of Immunology and Biochemistry, Danish Veterinary Institute, DK-1790 Copenhagen V

• K. Tjørnehøj

  Department of Immunology and Biochemistry, Danish Veterinary Institute, DK-1790 Copenhagen V

• F. Lampreave

  Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Ciencias, Universidad de Zaragoza, 50009 Zaragoza, Spain

• A. Piñeiro

  Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Ciencias, Universidad de Zaragoza, 50009 Zaragoza, Spain

• M. A. Alava

  Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Ciencias, Universidad de Zaragoza, 50009 Zaragoza, Spain

抄録

We have isolated from calf serum a protein with an apparent M r of 120,000. The protein was detected by using antibodies against major acute-phase protein in pigs with acute inflammation. The amino acid sequence of an internal fragment revealed that this protein is the bovine counterpart of ITIH4, the heavy chain 4 of the inter-alpha-trypsin inhibitor family. The response of this protein in the sera was determined for animals during experimental bacterial and viral infections. In the bacterial model, animals were inoculated with a mixture of Actinomyces pyogenes , Fusobacterium necrophorum , and Peptostreptococcus indolicus to induce an acute-phase reaction. All animals developed moderate to severe clinical mastitis and exhibited remarkable increases in ITIH4 concentration in serum (from 3 to 12 times the initial values, peaking at 48 to 72 h after infection) that correlated with the severity of the disease. Animals with experimental infections with bovine respiratory syncytial virus (BRSV) also showed increases in ITIH4 concentration (from two- to fivefold), which peaked at around 7 to 8 days after inoculation. Generally, no response was seen after a second infection of the same animals with the virus. Because of the significant induction of the protein in the animals in the mastitis and BRSV infection models, we can conclude that ITIH4 is a new positive acute-phase protein in cattle.

収録刊行物

• Infection and Immunity

  Infection and Immunity 72 (7), 3777-3782, 2004-07

  American Society for Microbiology

キーワード

• Infectious Diseases
• Immunology
• Microbiology
• Parasitology

詳細情報

• CRID: 1363951795228573824
• NII論文ID: 30020832457
• DOI: 10.1128/iai.72.7.3777-3782.2004
• ISSN: 10985522
• ISSN: 00199567
• Web Site: https://journals.asm.org/doi/pdf/10.1128/IAI.72.7.3777-3782.2004
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