Implications for Induction of Autoimmunity via Activation of B-1 Cells by<i>Helicobacter pylori</i>Urease

• Shingo Yamanishi

  Department of Microbiology and Immunology

• Tadasu Iizumi

  Department of Microbiology and Immunology

• Eri Watanabe

  Department of Microbiology and Immunology

• Masumi Shimizu

  Department of Microbiology and Immunology

• Shigeru Kamiya

  Department of Infectious Disease, Division of Medical Microbiology, Kyorin University School of Medicine, 6-20-2 Shinkawa, Mitaka, Tokyo 181-8611

• Kumiko Nagata

  Department of Bacteriology, Hyogo College of Medicine, 1-1 Mukogawa-cho, Nishinomiya, Hyogo 663-8131, Japan

• Yoshihiro Kumagai

  Department of Microbiology and Immunology

• Yoshitaka Fukunaga

  Department of Pediatrics, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo 113-8602

• Hidemi Takahashi

  Department of Microbiology and Immunology

抄録

Besides various gastroduodenal diseases, Helicobacter pylori infection may be involved in autoimmune disorders like rheumatoid arthritis (RA) or idiopathic thrombocytopenic purpura. Such autoimmune disorders are often associated with autoreactive antibodies produced by B-1 cells, a subpopulation of B lymphocytes. These B-1 cells are mainly located in the pleural cavity or mucosal compartment. The existence of H. pylori urease-specific immunoglobulin A (IgA)-producing B cells in the mucosal compartment and of their specific IgM in the sera of acutely infected volunteers suggests the possibility that urease stimulates mucosal innate immune responses. Here, we show for the first time that purified H. pylori urease predominantly stimulates the B-1-cell population rather than B-2 cells, which produce antigen-specific conventional antibodies among splenic B220 + B cells. The fact that such stimulation of B-1 cells was not affected by the addition of polymyxin B indicates that the effect of purified H. pylori urease was not due to the contamination with bacterial lipopolysaccharide. Furthermore, the production of various B-1-cell-related autoreactive antibodies such as IgM-type rheumatoid factor, anti-single-stranded DNA antibody, and anti-phosphatidyl choline antibody was observed when the splenic B cells were stimulated with purified H. pylori urease in vitro. These findings suggest that H. pylori components, urease in particular, may be among the environmental triggars that initiate various autoimmune diseases via producing autoreactive antibodies through the activation of B-1 cells. The findings shown here offer important new insights into the pathogenesis of autoimmune disorders related to H. pylori infection.

収録刊行物

• Infection and Immunity

  Infection and Immunity 74 (1), 248-256, 2006-01

  American Society for Microbiology

キーワード

• Infectious Diseases
• Immunology
• Microbiology
• Parasitology

詳細情報

• CRID: 1362262945400745472
• NII論文ID: 30020833069
• DOI: 10.1128/iai.74.1.248-256.2006
• ISSN: 10985522
• ISSN: 00199567
• Web Site: https://journals.asm.org/doi/pdf/10.1128/IAI.74.1.248-256.2006
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