Utilization of the Methoxymalonyl-Acyl Carrier Protein Biosynthesis Locus for Cloning the Oxazolomycin Biosynthetic Gene Cluster from <i>Streptomyces albus</i> JA3453

DOI Web Site 被引用文献3件
  • Chunhua Zhao
    Laboratory of Microbial Metabolism and School of Life Science and Biotechnology, Shanghai Jiaotong University, Shanghai 200030, China
  • Jianhua Ju
    Department of Chemistry, University of California, Davis, California 95616
  • Steven D. Christenson
    Department of Chemistry, University of California, Davis, California 95616
  • Wyatt C. Smith
    Department of Chemistry, University of California, Davis, California 95616
  • Danfeng Song
    Laboratory of Microbial Metabolism and School of Life Science and Biotechnology, Shanghai Jiaotong University, Shanghai 200030, China
  • Xiufen Zhou
    Laboratory of Microbial Metabolism and School of Life Science and Biotechnology, Shanghai Jiaotong University, Shanghai 200030, China
  • Ben Shen
    Department of Chemistry, University of California, Davis, California 95616
  • Zixin Deng
    Laboratory of Microbial Metabolism and School of Life Science and Biotechnology, Shanghai Jiaotong University, Shanghai 200030, China

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<jats:title>ABSTRACT</jats:title> <jats:p> Oxazolomycin (OZM), a hybrid peptide-polyketide antibiotic, exhibits potent antitumor and antiviral activities. Using degenerate primers to clone genes encoding methoxymalonyl-acyl carrier protein (ACP) biosynthesis as probes, a 135-kb DNA region from <jats:italic>Streptomyces albus</jats:italic> JA3453 was cloned and found to cover the entire OZM biosynthetic gene cluster. The involvement of the cloned genes in OZM biosynthesis was confirmed by deletion of a 12-kb DNA fragment containing six genes for methoxymalonyl-ACP biosynthesis from the specific region of the chromosome, as well as deletion of the <jats:italic>ozmC</jats:italic> gene within this region, to generate OZM-nonproducing mutants. </jats:p>

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