Detection of <i>Treponema denticola</i> in Atherosclerotic Lesions

• Katsuji Okuda

  <!--label omitted: 1-->Oral Health Science Center1 and

• Kazuyuki Ishihara

  <!--label omitted: 1-->Oral Health Science Center1 and

• Taneaki Nakagawa

  <!--label omitted: 1-->Oral Health Science Center1 and

• Akihiko Hirayama

  <!--label omitted: 1-->Oral Health Science Center1 and

• Yoshiyuki Inayama

  <!--label omitted: 4-->Departments of Anatomic and Surgical Pathology4 and

• Kenji Okuda

  <!--label omitted: 5-->Bacteriology,5 Yokohama City University School of Medicine, Yokohama, Japan

抄録

We examined 26 atherosclerotic lesions and 14 nondiseased aorta specimens to detect the periodontopathogenic part of the bacterial 16S rRNA locus by PCR. Treponema denticola sequence of the 16S rRNA locus was found in 6 out of 26 DNA samples (23.1%) from the formalin-fixed, paraffin-embeded atherosclerotic lesions obtained during surgery but not in any of the 14 nondiseased aorta samples from deceased persons. Utilizing immunofluorescence microscopy, we observed aggregated antigenic particles reacting with rabbit antiserum against T. denticola in thin sections of the PCR-positive samples, but we could not detect any reacting particles in the PCR-negative thin sections.

収録刊行物

• Journal of Clinical Microbiology

  Journal of Clinical Microbiology 39 (3), 1114-1117, 2001-03

  American Society for Microbiology

キーワード

• Microbiology (medical)

詳細情報

• CRID: 1360574093773370368
• NII論文ID: 30021102865
• DOI: 10.1128/jcm.39.3.1114-1117.2001
• ISSN: 1098660X
• ISSN: 00951137
• Web Site: https://journals.asm.org/doi/pdf/10.1128/JCM.39.3.1114-1117.2001
• データソース種別:
  • Crossref
  • CiNii Articles