Description of a New Yeast Species, <i>Malassezia japonica</i> , and Its Detection in Patients with Atopic Dermatitis and Healthy Subjects

• Takashi Sugita

  Department of Microbiology

• Masako Takashima

  Japan Collection of Microorganisms, Bioscience Technology Center, RIKEN (The Institute of Physical and Chemical Research), Wako, Saitama, Japan

• Minako Kodama

  Department of Immunobiology, Meiji Pharmaceutical University, Kiyose

• Ryoji Tsuboi

  Department of Dermatology, Tokyo Medical University, Shinjuku-ku, Tokyo

• Akemi Nishikawa

  Department of Immunobiology, Meiji Pharmaceutical University, Kiyose

抄録

Lipophilic yeasts of the genus Malassezia are part of the normal cutaneous microflora and are considered one of the factors that trigger atopic dermatitis (AD). We isolated two strains of Malassezia from a healthy Japanese female. Analysis of the D1/D2 26S ribosomal DNA and internal transcribed spacer region sequences of the isolates suggested that they are new members of the genus Malassezia . We propose the name Malassezia japonica sp. nov. for the isolates. M . japonica is easily distinguished from the seven known lipophilic species by its ability to assimilate Tween 40 and Tween 60 and its inability to assimilate Tween 20 and Tween 80 and to grow at 40°C. Furthermore, by applying transparent dressings to the skin lesions of 36 patients with AD and the skin of 22 healthy subjects, M . japonica DNA was detected by a non-culture-based method consisting of nested PCR with M . japonica species-specific primers. M . japonica DNA was detected from 12 of the 36 patients (33.3%) and 3 of the 22 healthy subjects (13.6%). Although it is not known whether M . japonica plays a role in AD, this species was part of the microflora in both patients with AD and healthy subjects.

収録刊行物

• Journal of Clinical Microbiology

  Journal of Clinical Microbiology 41 (10), 4695-4699, 2003-10

  American Society for Microbiology

キーワード

• Microbiology (medical)

詳細情報

• CRID: 1362262943676097920
• NII論文ID: 30021104517
• DOI: 10.1128/jcm.41.10.4695-4699.2003
• ISSN: 1098660X
• ISSN: 00951137
• Web Site: https://journals.asm.org/doi/pdf/10.1128/JCM.41.10.4695-4699.2003
• データソース種別:
  • Crossref
  • CiNii Articles
  • KAKEN