Use of Fatty Acid RPMI 1640 Media for Testing Susceptibilities of Eight <i>Malassezia</i> Species to the New Triazole Posaconazole and to Six Established Antifungal Agents by a Modified NCCLS M27-A2 Microdilution Method and Etest

DOI Web Site 被引用文献3件
  • Aristea Velegraki
    Mycology Reference Laboratory (Hellenic Centre for Diseases Control), Department of Microbiology, Medical School, University of Athens, Athens, Greece
  • Evangelos C. Alexopoulos
    Mycology Reference Laboratory (Hellenic Centre for Diseases Control), Department of Microbiology, Medical School, University of Athens, Athens, Greece
  • Stavroula Kritikou
    Mycology Reference Laboratory (Hellenic Centre for Diseases Control), Department of Microbiology, Medical School, University of Athens, Athens, Greece
  • George Gaitanis
    Mycology Reference Laboratory (Hellenic Centre for Diseases Control), Department of Microbiology, Medical School, University of Athens, Athens, Greece

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<jats:title>ABSTRACT</jats:title> <jats:p> A novel formulation of RPMI 1640 medium for susceptibility testing of <jats:italic>Malassezia</jats:italic> yeasts by broth microdilution (BMD) and Etest is proposed. A modification of the NCCLS M27-A2 BMD method was used to test 53 isolates of <jats:italic>Malassezia furfur</jats:italic> (12 isolates), <jats:italic>M. sympodialis</jats:italic> (8 isolates), <jats:italic>M. slooffiae</jats:italic> (4 isolates), <jats:italic>M. globosa</jats:italic> (22 isolates), <jats:italic>M. obtusa</jats:italic> (2 isolates), <jats:italic>M. restricta</jats:italic> (2 isolates), <jats:italic>M. pachydermatis</jats:italic> (1 isolates), and <jats:italic>M. dermatis</jats:italic> (2 isolates) against amphotericin B, ketoconazole, itraconazole, fluconazole, voriconazole, terbinafine, and posaconazole by BMD and Etest. RPMI and antibiotic medium 3 (AM3) were supplemented with glucose, bile salts, a mixture of fatty acids, and <jats:italic>n</jats:italic> -octadecanoate fatty acids and Tween 20. <jats:italic>M. furfur</jats:italic> ATCC 14521 and <jats:italic>M. globosa</jats:italic> ATCC 96807 were used as quality control strains. Depending on the species, MICs were read after 48 or 72 h of incubation at 32°C. Low azole and terbinafine MICs were recorded for all <jats:italic>Malassezia</jats:italic> species, whereas amphotericin B displayed higher MICs (≥16 μg/ml) against <jats:italic>M. furfur</jats:italic> , <jats:italic>M. restricta</jats:italic> , <jats:italic>M. globosa</jats:italic> , and <jats:italic>M. slooffiae</jats:italic> strains, which were AM3 confirmed. Agreement of the two methods was 84 to 97%, and intraclass correlation coefficients were statistically significant ( <jats:italic>P</jats:italic> < 0.001). Because of higher amphotericin B MICs provided by Etest for strains also displaying high BMD MICs (≥ <jats:bold>1</jats:bold> μg/ml), agreement was poorer. The proposed media are used for the first time and can support optimum growth of eight <jats:italic>Malassezia</jats:italic> species for recording concordant BMD and Etest MICs. </jats:p>

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