Effects of Tokishakuyakusan and Its Components on Rat Corpus Luteum Function <i>In Vitro</i>

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  • Satoshi Usuki
    Department of Obstetrics and Gynecology, Institute of Clinical Medicine, University of Tsukuba, Tennodail-I-I, Sakura-mura, Niihari-gun, Ibaraki 305, Japan

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<jats:p> 27-day-old female rats received 20 IU PMS and 56 hours later, 40 IU hCG. Seven days after hCG treatment, the resected ovaries were perifused with NIH-LH, Tokishakuyakusan (TJ-23) or its components. LH and TJ-23 increased progesterone in media and LH was more effective than TJ-23. Hoelen, Peony root, Alisma rhizome and Japanese angelica root also agumented progesterone. 17α-hydroxyprogesterone and 20 α-hydroxyprogesterone were not stimulated by TJ-23. These results suggest that TJ-23 stimulates corpora lutea to secrete progesterone without direct luteotropic or luteolytic effect and that the effect may be attributable to Hoelen, Peony root, Alisma rhizome or Japanese angelica root. </jats:p>

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