Effects of administration of progenipoietin 1, Flt-3 ligand, granulocyte colony-stimulating factor, and pegylated granulocyte-macrophage colony-stimulating factor on dendritic cell subsets in mice

DOI PDF 被引用文献5件
  • Meredith O'Keeffe
    From the Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia; Institute for Medical Microbiology, Immunology and Hygiene, Technical University of Munich, Germany; and Pharmacia, St Louis, MO.
  • Hubertus Hochrein
    From the Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia; Institute for Medical Microbiology, Immunology and Hygiene, Technical University of Munich, Germany; and Pharmacia, St Louis, MO.
  • David Vremec
    From the Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia; Institute for Medical Microbiology, Immunology and Hygiene, Technical University of Munich, Germany; and Pharmacia, St Louis, MO.
  • Joanne Pooley
    From the Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia; Institute for Medical Microbiology, Immunology and Hygiene, Technical University of Munich, Germany; and Pharmacia, St Louis, MO.
  • Robert Evans
    From the Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia; Institute for Medical Microbiology, Immunology and Hygiene, Technical University of Munich, Germany; and Pharmacia, St Louis, MO.
  • Susan Woulfe
    From the Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia; Institute for Medical Microbiology, Immunology and Hygiene, Technical University of Munich, Germany; and Pharmacia, St Louis, MO.
  • Ken Shortman
    From the Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia; Institute for Medical Microbiology, Immunology and Hygiene, Technical University of Munich, Germany; and Pharmacia, St Louis, MO.

抄録

<jats:title>Abstract</jats:title><jats:p>We studied the effects of administration of several cytokines, including progenipoietin-1 (ProGP-1), Flt-3 ligand (FL), granulocyte colony-stimulating factor (G-CSF), and granulocyte-macrophage colony-stimulating factor in a pegylated form (pGM-CSF), on dendritic cell (DC) populations in mouse spleen. ProGP-1 produced the most striking increase in overall DC numbers, apparently more than its constituent FL and G-CSF components. However, the expansion in DC numbers was strongly subpopulation selective, with ProGP-1 and FL producing selective expansion of CD8+ DCs, whereas pGM-CSF produced selective expansion of CD8− DCs. Surprising differences were observed between the effects of murine and human recombinant FL preparations on murine DCs. Many of the biologic functions of the DC subpopulations expanded by cytokines remained intact, including the capacity of the ProGP-1– and FL-expanded CD8+ DCs to produce the T-helper-1–biasing cytokine interleukin 12 (IL-12). However, the expanded DCs from all but G-CSF–treated mice were deficient in the ability to make interferon γ, and the CD8+ DCs produced with pGM-CSF treatment had an abrogated capacity to form bioactive IL-12. Such selective expansion of DC populations and alterations in their cytokine-secretion capacity have implications for clinical use of the studied cytokines in immune modulation.</jats:p>

収録刊行物

  • Blood

    Blood 99 (6), 2122-2130, 2002-03-15

    American Society of Hematology

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