Nifedipine Indirectly Upregulates Superoxide Dismutase Expression in Endothelial Cells via Vascular Smooth Muscle Cell–Dependent Pathways

• Keisuke Fukuo

  From Department of Geriatric Medicine, Osaka University Medical School, Suita, Osaka, Japan.

• Jin Yang

  From Department of Geriatric Medicine, Osaka University Medical School, Suita, Osaka, Japan.

• Osamu Yasuda

  From Department of Geriatric Medicine, Osaka University Medical School, Suita, Osaka, Japan.

• Masaki Mogi

  From Department of Geriatric Medicine, Osaka University Medical School, Suita, Osaka, Japan.

• Toshimitsu Suhara

  From Department of Geriatric Medicine, Osaka University Medical School, Suita, Osaka, Japan.

• Naoyuki Sato

  From Department of Geriatric Medicine, Osaka University Medical School, Suita, Osaka, Japan.

• Takashi Suzuki

  From Department of Geriatric Medicine, Osaka University Medical School, Suita, Osaka, Japan.

• Shigeto Morimoto

  From Department of Geriatric Medicine, Osaka University Medical School, Suita, Osaka, Japan.

• Toshio Ogihara

  From Department of Geriatric Medicine, Osaka University Medical School, Suita, Osaka, Japan.

抄録

Background — Calcium antagonists normalize endothelial dysfunction in many cardiovascular diseases. There is no known receptor, however, for calcium antagonists in endothelial cells (ECs). We hypothesized that vascular smooth muscle cells (VSMCs) are involved in the mechanism underlying the normalization of endothelial dysfunction by calcium antagonists. Methods and Results — Coculture studies with ECs and VSMCs were performed to determine whether VSMCs mediate modulation of endothelial superoxide dismutase (SOD) activity and expression induced by the calcium antagonist nifedipine. Nifedipine induced upregulation of SOD activity in rat aortic segments but had no effect on SOD expression or activity in ECs or VSMCs cultured individually. When ECs were cocultured with VSMCs, however, nifedipine upregulated SOD expression and activity in ECs. Nifedipine stimulated vascular endothelial growth factor (VEGF) production from VSMCs, and this stimulation of VEGF production was abolished by HOE-140, an antagonist of the bradykinin B 2 receptor. A neutralizing antibody against VEGF inhibited the upregulation of endothelial SOD by nifedipine. In addition, recombinant VEGF induced an increase in the levels of SOD expression in ECs, and supernatant derived from nifedipine-treated VSMCs enhanced NO production from ECs. This increase in NO production by the supernatant was inhibited by preincubation of ECs with SOD antisense oligodeoxyribonucleotides. Conclusions — The calcium antagonist nifedipine indirectly upregulates endothelial SOD expression by stimulating VEGF production from adjacent VSMCs. This finding may provide further insight into the mechanism underlying the beneficial effects of calcium antagonists in cardiovascular diseases.

収録刊行物

• Circulation

  Circulation 106 (3), 356-361, 2002-07-16

  Ovid Technologies (Wolters Kluwer Health)

キーワード

• Physiology (medical)
• Cardiology and Cardiovascular Medicine

詳細情報

• CRID: 1363107369148289152
• NII論文ID: 30022667260
• DOI: 10.1161/01.cir.0000021924.02006.ba
• ISSN: 15244539
• ISSN: 00097322
• Web Site: https://www.ahajournals.org/doi/full/10.1161/01.CIR.0000021924.02006.BA
• データソース種別:
  • Crossref
  • CiNii Articles
  • KAKEN