Comparative phylogenies of the housekeeping genes atpD, infB and rpoB and the 16S rRNA gene within the Pasteurellaceae

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  • Henrik Christensen
    Department of Veterinary Microbiology, Stigbøjlen 4, Royal Danish Veterinary and Agricultural University, 1870 Frederiksberg C, Denmark
  • Peter Kuhnert
    Institute of Veterinary Bacteriology, University of Bern, Laenggass-Strasse 122, CH-3012 Bern, Switzerland
  • John Elmerdahl Olsen
    Department of Veterinary Microbiology, Stigbøjlen 4, Royal Danish Veterinary and Agricultural University, 1870 Frederiksberg C, Denmark
  • Magne Bisgaard
    Department of Veterinary Microbiology, Stigbøjlen 4, Royal Danish Veterinary and Agricultural University, 1870 Frederiksberg C, Denmark

抄録

<jats:p>Phylogenies of housekeeping gene and 16S rRNA gene sequences were compared to improve the classification of the bacterial family <jats:italic>Pasteurellaceae</jats:italic> and knowledge of the evolutionary relationships of its members. Deduced partial protein sequences of the housekeeping genes <jats:italic>atpD</jats:italic>, <jats:italic>infB</jats:italic> and <jats:italic>rpoB</jats:italic> were compared in 28, 36 and 28 representative taxa of the <jats:italic>Pasteurellaceae</jats:italic>, respectively. The monophyly of representatives of the genus <jats:italic>Gallibacterium</jats:italic> was recognized by analysis of all housekeeping genes, while members of <jats:italic>Mannheimia</jats:italic>, <jats:italic>Actinobacillus sensu stricto</jats:italic> and the core group of <jats:italic>Pasteurella sensu stricto</jats:italic> formed monophyletic groups with two out of three housekeeping genes. Representatives of <jats:italic>Mannheimia</jats:italic>, <jats:italic>Actinobacillus sensu stricto</jats:italic>, [<jats:italic>Haemophilus</jats:italic>] <jats:italic>ducreyi</jats:italic> and [<jats:italic>Pasteurella</jats:italic>] <jats:italic>trehalosi</jats:italic> formed a monophyletic unit by analysis of all three housekeeping genes, which was in contrast to the 16S rRNA gene-derived phylogeny, where these taxa occurred at separate positions in the phylogenetic tree. Representatives of the Rodent, Avian and Aphrophilus–<jats:italic>Haemophilus</jats:italic> 16S rRNA gene groups were weakly supported by phylogenetic analysis of housekeeping genes. Phylogenies derived by comparison of the housekeeping genes diverged significantly from the 16S rRNA gene-derived phylogeny as evaluated by the likelihood ratio test. A low degree of congruence was also observed between the individual housekeeping gene-derived phylogenies. Estimates on speciation derived from 16S rRNA and housekeeping gene sequence comparisons resulted in quite different evolutionary scenarios for members of the <jats:italic>Pasteurellaceae</jats:italic>. The phylogeny based on the housekeeping genes supported observed host associations between <jats:italic>Mannheimia</jats:italic>, <jats:italic>Actinobacillus sensu stricto</jats:italic> and [<jats:italic>Pasteurella</jats:italic>] <jats:italic>trehalosi</jats:italic> and animals with paired hooves.</jats:p>

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