Two Potato Proteins, Including a Novel RING Finger Protein (HIP1), Interact with the Potyviral Multifunctional Protein HCpro

• Deyin Guo

• Carl Spetz

• Mart Saarma

• Jari P. T. Valkonen

抄録

Potyviral helper-component proteinase (HCpro) is a multifunctional protein exerting its cellular functions in interaction with putative host proteins. In this study, cellular protein partners of the HCpro encoded by Potato virus A (PVA) (genus Potyvirus) were screened in a potato leaf cDNA library using a yeast two-hybrid system. Two cellular proteins were obtained that interact specifically with PVA HCpro in yeast and in the two in vitro binding assays used. Both proteins are encoded by single-copy genes in the potato genome. Analysis of the deduced amino acid sequences revealed that one (HIP1) of the two HCpro interactors is a novel RING finger protein. The sequence of the other protein (HIP2) showed no resemblance to the protein sequences available from databanks and has known biological functions.

収録刊行物

• Molecular Plant-Microbe Interactions®

  Molecular Plant-Microbe Interactions® 16 (5), 405-410, 2003-05

  Scientific Societies

キーワード

• Agronomy and Crop Science
• General Medicine
• Physiology

詳細情報

• CRID: 1361418518999071232
• NII論文ID: 30027612358
• DOI: 10.1094/mpmi.2003.16.5.405
• ISSN: 19437706
• ISSN: 08940282
• Web Site: https://apsjournals.apsnet.org/doi/pdf/10.1094/MPMI.2003.16.5.405
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  • CiNii Articles