Amperometric Alcohol-Sensing Electrode Based on a Polyion Complex Membrane Containing Alcohol Oxidase.
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- MIZUTANI Fumio
- National Institute of Bioscience and Human-Technology
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- YABUKI Soichi
- National Institute of Bioscience and Human-Technology
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- IIJIMA Seiichiro
- National Institute of Bioscience and Human-Technology
書誌事項
- タイトル別名
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- Amperometric Alcohol-Sensing Electrode
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An amperometric alcohol-sensing electrode was prepared by immobilizing alcohol oxidase (AOx) in a polyion complex membrane. Three kinds of aqueous solutions (poly(4-styrenesulfonate)-, AOx- and poly-L-lysine-containing solutions) were successively placed on the surface of a glassy carbon electrode and the electrode was allowed to dry. The anodic current (at 1V vs. Ag/AgCl) of the enzyme electrode increased immediately after the addition of alcohols (e.g., ethanol), and reached a steady state within 5s. A linear response to ethanol was observed up to 0.4mM with a detection limit of 0.5μM. The polyion complex membrane showed permselectivity based on the solute size with a molecule weight cut-off of ca. 100, which was very effective in suppressing the electrochemical interference by L-ascorbic acid and uric acid. The enzyme electrode was applied to the determination of ethanol in alcoholic drinks and soy sources. The electrode could be used for two weeks.
収録刊行物
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- Analytical Sciences
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Analytical Sciences 13 (1), 83-87, 1997
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390001204258467328
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- NII論文ID
- 130003528138
- 30031575140
- 10002410591
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- NII書誌ID
- AA10500785
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- COI
- 1:CAS:528:DyaK2sXht1KjtLs%3D
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- ISSN
- 13482246
- 09106340
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- NDL書誌ID
- 4142761
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- 使用不可