Lymphocyte Metallothionein-mRNA as a Sensitive Biomarker of Cadmium Exposure.
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- YAMADA Hirotomo
- Division of Health Effects Research
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- KOIZUMI Shinji
- Division of Hazard Assessment, National Institute of Industrial Health
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Human peripheral blood lymphocytes (PBLs) produce metallothioneins (MTs) in response to a variety of heavy metal ions. MTs could therefore be a candidate for a marker that represents the biological effect of heavy metals. Since it is practically difficult to measure MT protein levels in PBLs, we examined if MT-mRNA could serve as a biomarker of heavy metal exposure. It is difficult to obtain RNA from PBLs without degradation, but we found that intact RNA can be prepared by the acid guanidinium thiocyanate-phenol-chloroform extraction method which minimizes nuclease digestion of RNA during purification steps. By Northern blot analysis of RNA isolated by this method from PBLs cultured with or without CdSO4, we demonstrated that MT-mRNA is induced by 0.1 to 0.5 μM CdSO4 in a dose-dependent manner. The blood cadmium levels of exposed humans have been reported to be up to 0.5 μM. This suggests that our assay is able to detect quantitative changes in the PBL MT-mRNA level resulting from in vivo cadmium exposure. Thus, PBL MT-mRNA could be used as a sensitive biomarker reflecting exposure to cadmium, and probably to several other MTinducing heavy metals.
収録刊行物
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- Industrial health
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Industrial health 39 (1), 29-32, 2001
独立行政法人 労働者健康安全機構 労働安全衛生総合研究所
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詳細情報 詳細情報について
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- CRID
- 1390001204281024640
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- NII論文ID
- 30035022312
- 130003550976
- 40005265714
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- NII書誌ID
- AA00672955
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- COI
- 1:CAS:528:DC%2BD3MXhtlyit74%3D
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- ISSN
- 18808026
- 00198366
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- NDL書誌ID
- 5994913
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- PubMed
- 11212288
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可