Identification, mapping, and application of polymorphic DNA associated with resistance gene <i>Pm21</i> of wheat

DOI Web Site 被引用文献2件

この論文をさがす

抄録

<jats:p> A new powdery mildew resistance gene designated Pm21, from Haynaldia villosa, a relative of wheat, has been identified and incorporated into wheat through an alien translocation line. Cytogenetic and biochemical analyses showed that chromosome arms 6VS and 6AL were involved in this translocation. Random amplified polymorphic DNA (RAPD) analysis was performed on recipient wheat cultivar Yangmai 5, the translocation line, and H. villosa with 180 random primers. Eight of the 180 primers amplified polymorphic DNA in the translocation line, and the same results were obtained in four replications. Furthermore, RAPD analysis was reported for substitution line 6V, seven addition lines (1V–7V), and the F<jats:sub>1</jats:sub>, as well as F<jats:sub>2</jats:sub> plants of (translocation line × 'Yangmai 5'), using two of the eight random primers. One RAPD marker, specific to chromosome arm 6VS, OPH17-1900, could be used as a molecular marker for the detection of gene Pm21 in breeding materials with powdery mildew resistance introduced from H. villosa. Key words : RAPD analysis, 6VS-specific marker, Pm21, Erysiphe graminis f.sp. tritici, Triticum aestivum – Haynaldia villosa translocation. </jats:p>

収録刊行物

  • Genome

    Genome 39 (1), 191-197, 1996-02-01

    Canadian Science Publishing

被引用文献 (2)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ