Immunohistochemical Studies of Plasma Protein-Producing Cells in Rat Liver. I. Influence of Various Fixatives and Fixation Procedures on the Demonstration of Albumin and Fibrinogen-Producine Cells in Normal Rat Liver

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  • 肝の血漿蛋白産生細胞に関する免疫組織化学的研究　Ⅰ.　種々の固定条件における正常ラット肝のAlbumin, Fibrinogen産生細胞の検索
  • 肝の血漿蛋白産生細胞に関する免疫組織化学的研究-1-種々の固定条件における正常ラット肝のAlbumin,Fibrinogen産生細胞の検索
  • カン ノ ケッショウ タンパク サンセイ サイボウ ニ カンスル メンエキ ソ

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Abstract

The influence of various fixation procedures and fixatives on the cellular and subcellular localization of albumin （ALB）, transferrin （Tf） and fibrinogen （Fg） in normal rat liver was examined by the immunoperoxidase technique. When the liver was fixed by perfusion through the portal vein with 8% paraformaldehyde, virtually all hepatocytes in the liver were stained simultaneously by the anti-ALB and anti-Tf anti-sera. On the other hand, hepatocytes positive for ALB and Tf were found only scattered when the liver was fixed by immersion. Since the positively stained hepatocytes for these proteins decreased in number parallel to the depth of the tissue from the surface of the specimen, it was strongly suggested that the outflux of intracellular proteins during the process of fixation would probably take place due to slow penetration of the fixatives. Fg was not found in the normal liver fixed by perfusion, suggesting that the content of this protein in normal hepatocytes is lower than the level detectable by the Immunoperoxidase technique. ALB and Tf were demonstrated to be clearly localized on the rough endoplasmic reticulum, nuclear envelope and the Golgi apparatus by immuno-electron microscopy specific for each protein.

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