後部絹糸腺フィブロインより調製した結晶部ペプチドの性質について

DOI 日本農学文献記事索引 Web Site 被引用文献3件
  • 片方 陽太郎
    Laboratory of Biochemistry, Department of Agricultural Chemistry, Tohoku University present address: Department of Dermatology, Yamagata University School of Medicine
  • 菊地 愛子
    Laboratory of Biochemistry, Department of Agricultural Chemistry, Tohoku University
  • 志村 憲助
    Laboratory of Biochemistry, Department of Agricultural Chemistry, Tohoku University

書誌事項

タイトル別名
  • Characterization of the crystalline-region peptides prepared from the posterior silk gland fibroin.
  • 後部絹糸腺フィブロインより調製した結晶部ペプチドの性質について〔英文〕
  • コウブ キヌイトセン フィブロイン ヨリ チョウセイシタ ケッショウブ ペプチド

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抄録

Crystalline-region peptides (Cp) of silk fibroin were prepared by α-chymotryptic digestion from the large subunit of fibroin, which was prepared from the posterior silk glands of Bombyx mori with cold 30% ethanol treatment. The ratio of crystalline to amorphous regions in the large subunit was about 55 to 45 in terms of amino acid residues. Sephadex G-50 gel filtration gave a single peak with a slight shoulder, indicating the molecular size of Cp lies within a fairly limited range. On the other hand, elution profile of Cp from a column of DEAE-Sephadex A-50 exhibited one main peak with a shoulder and three minor peaks. This result shows the heterogeneity of Cp peptides in respect to their electrostatic charges. Amino acid analysis of these peptide fractions also supports this opinion. Molecular weight of Cp was estimated to be about 3900-4100 from terminal analyses and amino acid composition.

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