マウスの胃腸内分泌細胞におけるL-DOPAとL-ロイシンおよびそれらの代謝産物の取込みと細胞内の局在に関する電顕オートラジオグラフィーによる研究

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  • Uptake and Intracellular Localization of Exogenous L-DOPA, L-Leucine and their Metabolites in the Gastro-Enteric Endocrine Cells of the Mouse Studied by Electron Microscope Autoradiography
  • マウス ノ イチョウ ナイブンピ サイボウ ニ オケル L ロイシン オヨビ

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L-DOPA-3H (50μCi/g.b.w.) was injected intraperitoneally in two mice. They were killed 1hr and 24hrs after the injection and pieces of the gut were processed for both light and electron microscope autoradiography after fixation in glutaraldehyde followed by post-fixation with osmium tetroxide. At 1hr, a greater number of specific silver grains were found on EC cells than on other types of basal-granulated cells including G, L (EG) and ECL cells. At 24hrs, a considerable number of labels were found on only a few EC cells. Subcellular localization of L-DOPA-3H and its metabolites was studied by electron microscope autoradiography. By 1hr after the injection, specific radioactivity accumulated in the cytoplasm rich in secretory granules. Labeling on the Golgi area was conspicuously lower than in the granule-rich cytoplasm.<br>To study the process of the synthesis of secretory proteins, L-leucine-3H (100μCi/g.b.w.) was injected into the peritoneal cavity of two mice which were killed at 30mins and 2.5hrs after the injection. It was apparent that all morphologic types of basal-granulated cells had an ability to uptake exogenous leucine to synthesize proteins/polypeptides. At 30mins, specific silver grains were found primarily on the Golgi area. By 2.5hrs, however, silver grains were mainly associated with the secretory granules and frequently conglomerated to form a cluster of them in the cytoplasmic area rich in secretory granules.<br>These results are consistent with the view that in all types of basal-granulated cells the secretory granules together with their protein contents are formed in the Golgi area, and then incorporate amines from the cytosol to become ready to be released by exocytosis.

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