On a New Continuous Cell Line (TM-1) Derived from Malignant Melanoma

DOI Web Site Web Site PubMed
  • NISHIHIRA TETSURO
    Department of Surgery, Tohoku University School of Medicine
  • KURIYA YOSHIKI
    Department of Surgery, Tohoku University School of Medicine
  • HAYASHI YUTAKA
    Department of Surgery, Tohoku University School of Medicine
  • ISHII HIROSHI
    Department of Surgery, Tohoku University School of Medicine
  • KIMURA MICHIO
    Department of Surgery, Tohoku University School of Medicine
  • TAN MASAKI
    Department of Surgery, Tohoku University School of Medicine
  • SASAKI TAKASHI
    Department of Surgery, Tohoku University School of Medicine
  • KASAI MORIO
    Department of Surgery, Tohoku University School of Medicine
  • KATOH TAIZO
    Department of Dermatology, Tohoku University School of Medicine
  • MATSUYA YUTAKA
    Research Institute for Tuberculosis and Cancer, Tohoku University
  • YAMANE ISAO
    Research Institute for Tuberculosis and Cancer, Tohoku University

書誌事項

タイトル別名
  • On a New Continuous Cell Line TM-1 Deri

この論文をさがす

抄録

A continuous cell line of malignant melanoma has been established. The tumor specimen was obtained from a left axillary lymph node of a malignant melanoma originating in the abdominal skin of a 67-year-old woman. The cell line has been designated TM-1. Explant specimens showed positive dopa staining, but after 2 years and 4 months, or 60 generations of cell passage, the dopa staining is now weakly positive. Treatment with DBcAMP that causes marked morphological changes induces melanin production and lowers the proliferative ability of the cells. These changes were reversible. Transplantation of the cells to nude mice resulted in the formation of large tumors consisting of epithelioid cells and spindle cells. Morphologically, the majority of the cell population in vitro was polygonal epithelioid cells, but the cell passage produced a large number of spindle cells. The shape of the TM-1 cells was examined by scanning electron microscopy. Depending upon the culture conditions, either of these cell types could be predominated, indicating that both cells are of the same origin.

収録刊行物

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ