Cultivation of V79 cells in serum-free medium containing bovine serum albumin and the use of gelatin as an attachment factor.

DOI Web Site Web Site
  • UMEDA MAKOTO
    Tissue Culture Laboratory, Yokohama City University School of Medicine Tokyo Metropolitan Institute of Medical Sciences
  • NODA KOUICHI
    Tissue Culture Laboratory, Yokohama City University School of Medicine
  • TANAKA KUMIKO
    Tokyo Metropolitan Institute of Medical Sciences
  • YAMANE ISAO
    Cell Biology Division, the Research Institute for Tuberculosis and Cancer, Tohoku University
  • MATSUYA YUTAKA
    Cell Biology Division, the Research Institute for Tuberculosis and Cancer, Tohoku University

書誌事項

タイトル別名
  • Cultivation of V79 Cells in Serum Free

この論文をさがす

抄録

UMEDA, M., NODA, K., TANAKA, K., YAMANE, I. and MATSUYA, Y. Cultivation of V79 Cells in Serum-Free Medium Containing Bovine Serum Albumin and the Use of Gelatin as an Attachment Factor. Tohoku J. exp. Med., 1983, 141 (1) 99-106 -V79 cells were cultivated in a serum-free medium. When a serum-free, bovine serum albumin-containing medium was used, removal of trypsin after cell detachment during subcultivation was necessary for routine mass cultivation. For colony formation addition of an attachment factor to the medium was further necessary. Gelatin was proved to serve as a good attachment factor in the colony formation and it could be substituted for fibronectin as an attachment factor for serum-free cultivation of many types of cells.

収録刊行物

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ