Discrimination of the length of double-stranded DNA fragments by the bis-intercalating ligand.

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  • TAKENAKA Shigeori
    Department of Chemical Systems and Engineering, Graduate School of Engineering, Kyushu University
  • FUNATU Yosuke
    Department of Biochemical Engineering and Science, Kyushu Institute of Technology
  • SHIGEMOTO Naonori
    Department of Biochemical Engineering and Science, Kyushu Institute of Technology
  • KONDO Hiroki
    Department of Biochemical Engineering and Science, Kyushu Institute of Technology

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タイトル別名
  • Discrimination of the Length of Double-

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Bis-intercalator 2 with two acridine moieties separated by a bis(hexamethylene)viologen linker was synthesized and its binding of calf thymus DNA was studied. Bis-intercalator 2 was designed to cover about a 24 Å stretch of double stranded DNA. The binding constant of bis-intercalator 2 for calf thymus DNA was 1.7x105M-1 in 10mM MES, 1mM EDTA and 0.1M NaCl (pH 6.25). The two acridine units of 2 stacked with each other in solution and upon its binding the structure of DNA was perturbed considerably. The binding affinities of the bis-intercalator for DNA duplex fragments of different length, d(AT)n where n=3-8, were estimated by its effect on the DNA melting temperature. It was found that d(AT)5 with a length of 34 Å was stabilized most by 2. By contrast, quinacrine 1 did not show any stabilizing effect on these DNAs. These results suggest that the bis-intercalator was capable of discriminating the length of double-stranded DNA fragments precisely. This ability of 2 may be applied for the precise separation of double-stranded DNA fragments according to their length.

収録刊行物

  • Analytical Sciences

    Analytical Sciences 13 (Supplement), 177-180, 1997

    社団法人 日本分析化学会

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