Investigation of the Purification/Concentration Method for Dioxins Analysis by GC-MS and Bioassay

• FUJITA Hiroyuki

  Enviromental Science for Industry, Ehime University

• HAMADA Noriaki

  Miura Institute of Environmental Science, MIURA Co., Ltd

• SAWADAISHI Kazuyuki

  Environmental Bioanalytical Chemistry, Ehime University

• HONDA Katsuhisa

  Enviromental Science for Industry, Ehime University

Bibliographic Information

Other Title

• ＧＣ‐ＭＳ法及び生物検定法によるダイオキシン類測定のための簡易精製・濃縮法に関する検討
• GC MSホウ オヨビ セイブツ ケンテイホウ ニ ヨル ダイオキシンルイ ソクテイ ノ タメ ノ カンイ セイセイ ノウシュクホウ ニ カンスル ケントウ

Abstract

Our previous report showed that the heating (60°C) multilayer silica gel columns, i.e., AgNO₃silica gel and H₂SO₄silica gel, was useful as purification method for dioxins analysis. In this study, further simplified-and automated-preparation method for dioxins analysis using HRGC-HRMS and Bioassay was studied on three successive steps of the purification, concentration and solvent substitution.<BR>The results showed the best suitable condition as follow: An alumina column as concentration and solvent substitution was set under multilayer silica gel column. Dioxins sample in hexane was applied on a top of multilayer silica gel column. After heating the column at 60°C, dioxins were eluted with 60 mlofn-Hexane kept at 60°C, and then followed by drying the alumina column with N₂gas. Thereafter, dioxins in alumina column were reversibly eluted with 0.5 mlof Toluene or DMSO solution kept at 60°C, and determined by GC-MS or Bioassay. This newly developed method for dioxins analysis was considered to be useful for reduction of analytical time, amount of solvent, and cost.

Journal

• Journal of Environmental Chemistry

  Journal of Environmental Chemistry 15 (3), 585-596, 2005

  Japan Society for Environmental Chemistry

Keywords

• Dioxins
• Automated solvent concentration
• Automated solvent substitution
• GC-MS
• Bioassay

Details

• CRID: 1390282679347702528
• NII Article ID: 130004162814
• NII Book ID: AN10547099
• DOI: 10.5985/jec.15.585
• ISSN: 18825818
• ISSN: 09172408
• NDL BIB ID: 7490657
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7490657
• Web Site: http://www.jstage.jst.go.jp/article/jec1991/15/3/15_3_585/_pdf
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed