Investigation of a Tyrosinase-Immobilized Polyacrylamide Membrane Electrode in <i>N</i>,<i>N</i>-Dimethylacetamide

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  • Investigation of a Tyrosinase-Immobilized Polyacrylamide Membrane Electrode in N,N-Dimethylacetamide

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A tyrosinase-immobilized, hydrophilic polyacrylamide membrane electrode was used to investigate the enzymatic reaction of catechol derivatives in DMA and the apparent Michaelis–Menten constants, Km,app, were determined. The method can be used for both monitoring enzymatic activity and solvent effect using only a small amount of enzyme.

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