Rapid multiplex immunofluorescent assay to detect antibodies against Burkholderia pseudomallei and taxonomically closely related nonfermenters

DOI Web Site Web Site
  • Iihara Hirotoshi
    Department of Microbiology, Regeneration and Advanced Medical Science, Gifu University Graduate School of Medicine, Japan Department of Pharmacy, Gifu University Hospital, Japan
  • Niwa Takashi
    Department of Microbiology, Regeneration and Advanced Medical Science, Gifu University Graduate School of Medicine, Japan Department of Pharmacy, Gifu University Hospital, Japan
  • Shah Mohammad Monir
    Department of Microbiology, Regeneration and Advanced Medical Science, Gifu University Graduate School of Medicine, Japan
  • Nhung Pham Hong
    Department of Microbiology, Regeneration and Advanced Medical Science, Gifu University Graduate School of Medicine, Japan
  • Sun Xiao Song
    Department of Microbiology, Regeneration and Advanced Medical Science, Gifu University Graduate School of Medicine, Japan
  • Hayashi Masahiro
    Department of Microbiology, Regeneration and Advanced Medical Science, Gifu University Graduate School of Medicine, Japan
  • Ohkusu Kiyofumi
    Department of Microbiology, Regeneration and Advanced Medical Science, Gifu University Graduate School of Medicine, Japan
  • Itoh Yoshinori
    Department of Pharmacy, Gifu University Hospital, Japan Department of Clinical Pharmacy, Gifu University Graduate School of Medicine, Japan
  • Makino Sou-ichi
    Laboratory of Food Microbiology and Immunology, Research Center for Animal Hygiene and Food Safety, Obihiro University of Agriculture and Veterinary Medicine, Japan
  • Ezaki Takayuki
    Department of Microbiology, Regeneration and Advanced Medical Science, Gifu University Graduate School of Medicine, Japan

書誌事項

タイトル別名
  • Rapid Multiplex Immunofluorescent Assay to Detect Antibodies against <i>Burkholderia pseudomallei</i> and Taxonomically Closely Related Nonfermenters

この論文をさがす

抄録

<p>An indirect immunofluorescent assay to detect antibodies against the lipopolysaccharide (LPS) of Burkholderia pseudomallei and taxonomically closely related species was developed with the Luminex system. LPSs of Pseudomonas aeruginosa, Burkholderia cepacia, Burkholderia thailandensis, Burkholderia vietnamiensis, B. pseudomallei, and Burkholderia mallei were successfully conjugated to Luminex microspheres. Antibodies measured against the LPS of B. pseudomallei-conjugated Luminex beads only cross-reacted with those of two genetically closely related species, B. mallei and B. thailandensis (previously classified as non-pathogenic arabinose-negative B. pseudomallei). However, this system could distinguish other closely related species from B. pseudomallei. This assay is able to detect significantly high levels of anti-LPS antibodies of B. pseudomallei in serum from patients with culture-proven melioidosis.</p>

収録刊行物

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ