Involvement of Choline Transporter-Like Proteins, CTL1 and CTL2, in Glucocorticoid-Induced Acceleration of Phosphatidylcholine Synthesis via Increased Choline Uptake

• Nakamura Toshimichi

  Department of Membrane Transport and Pharmacokinetics, Faculty of Pharmacy, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University Faculty of Pharmaceutical Sciences, Tokyo University of Science

• Fujiwara Ryohei

  Faculty of Pharmaceutical Sciences, Tokyo University of Science

• Ishiguro Naoki

  Faculty of Pharmaceutical Sciences, Tokyo University of Science

• Oyabu Masanobu

  Faculty of Pharmaceutical Sciences, Tokyo University of Science

• Nakanishi Takeo

  Department of Membrane Transport and Pharmacokinetics, Faculty of Pharmacy, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University

• Shirasaka Yoshiyuki

  Department of Membrane Transport and Pharmacokinetics, Faculty of Pharmacy, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University Faculty of Pharmaceutical Sciences, Tokyo University of Science

• Maeda Tomoji

  Department of Membrane Transport and Pharmacokinetics, Faculty of Pharmacy, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University Department of Neuroscience, Faculty of Pharmaceutical Sciences, Iwate Medical University

• Tamai Ikumi

  Department of Membrane Transport and Pharmacokinetics, Faculty of Pharmacy, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University Faculty of Pharmaceutical Sciences, Tokyo University of Science

抄録

Phosphatidylcholine (PC) production is accelerated by glucocorticoid, such as dexamethasone (DEX), which enhances fetal lung maturation, promotes differentiation of alveolar type II (ATII) cells, and increases production of both lipid and protein components of lung surfactant. We previously demonstrated that inhibition of choline uptake by ATII cells leads to a decrease of PC synthesis. Since choline uptake may play a critical role in PC production and lung surfactant homeostasis for normal breathing, it is of interest to characterize transporters controlling the disposition of choline in ATII cells. Therefore, we studied the gene regulation and activity of choline transporters in A549 cells, a human ATII cell line. A549 cells were exposed to DEX for 24 h, and mRNA expression levels of choline transporters-like protein 1, (CTL1) and CTL2, were measured using real-time reverse transcription polymerase chain reaction. CTL1 and CTL2 mRNAs were strongly induced by DEX treatment of A549 cells, and the DEX-treated cells showed a significant increase in initial uptake rate of [³H]choline, which was assessed under ATP-depleted conditions to block the influence of consumption of choline by choline kinase. Transfection of A549 cells with either CTL1- or CTL2-small interfering RNAs significantly decreased [³H]choline uptake. In conclusion, choline transport in A549 cells is increased by treatment with DEX, and the increase is mediated by induction of functional choline transporters CTL1 and CTL2.

収録刊行物

• Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ

  Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ 33 (4), 691-696, 2010

  公益社団法人 日本薬学会

キーワード

• choline transporter
• alveolar type II cell
• dexamethasone
• glucocorticoid receptor
• lung surfactant

詳細情報

• CRID: 1390282679604863488
• NII論文ID: 40017031070
• NII書誌ID: AA10885497
• DOI: 10.1248/bpb.33.691
• ISSN: 13475215
• ISSN: 09186158
• NDL書誌ID: 10622856
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10622856
• Web Site: http://www.jstage.jst.go.jp/article/bpb/33/4/33_4_691/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2010223468
• 本文言語コード: en
• データソース種別:
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  • NDL
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  • CiNii Articles
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