Detection of <i>Anaplasma bovis</i> DNA in the Peripheral Blood of Domestic Dogs in Japan

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  • Detection of Anaplasma bovis DNA in the peripheral blood of domestic dogs in Japan
  • Detection of Anaplasma bovis DNA from peripheral blood of domestic dogs in Japan

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Abstract

<p>The prevalence of Ehrlichia and Anaplasma in 1,427 dogs from 32 Japanese prefectures was evaluated by PCR and DNA nucleotide sequencing. PCR screening demonstrated that 18 dogs (1.3%) were positive for Anaplasmataceae. Sequence analysis revealed that 14 of the amplicons were most closely related to Wolbachia spp., symbionts of Dirofilaria immitis, whereas three were identified as Anaplasma bovis. The remaining amplicon could not be sequenced. Almost the entire sequence of 16S rRNA (1,452 bp) from one of the positive specimens was determined, and subsequent phylogenetic analysis confirmed that the detected sequence was that of A. bovis. This is the first detection of A. bovis DNA fragments in dogs. Species-specific nested PCR showed that 15 (1.1%) of the 1,427 dogs involved in this study were positive for A. bovis. The geographical distribution of these dogs ranged from Aomori Prefecture in northern Japan to Kagoshima Prefecture in the south. The relationship between A. bovis infection and clinical disease is not yet clearly understood.<tt> </tt></p>

Journal

  • Japanese Journal of Infectious Diseases

    Japanese Journal of Infectious Diseases 63 (5), 349-352, 2010-09-30

    National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee

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