ヒラメ滑走細菌症の浸漬法による実験感染条件について

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タイトル別名
  • Evaluation of an Experimental Immersion Infection Method with <I>Tenacibaculum maritimum</I> in Japanese Flounder <I>Paralichthys olivaceus</I>
  • Evaluation of an experimental immersion infection method with Tenacibaculum maritimum in Japanese flounder Paralichthys olivaceus

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抄録

Necessary condition to perform experimental infection with Tenacibaculum maritimum was investigated. A shaking culture of the bacterium for 48 h was used to infect Japanese flounder Paralichthys olivaceous. First, three infection methods, intraperitoneal (IP) injection and two immersion methods were compared. IP injected flounder with T. maritimum at doses of 104.82 or 104.92 CFU/fish did not exhibit high mortality or typical symptoms. “Immersion and dilution method”, where immersion-infected fish at doses 105.81 or 105.91 CFU/ml were successively reared in the same tank supplying fresh rearing water, resulted in higher mortality than “immersion and transfer method”, where immersion-infected fish were transferred to another rearing tank. Using immersion and dilution method, stable mortality occurred between 17°C and 26°C but not below 17°C or over 26°C. Too much diluted bacterial culture with sea water did not yield high mortality even when the immersion water contained enough bacterial concentration. This suggests extracellular substance contains virulence factor. T. maritimum was detected on the body surface of infected fish but not in the gills or kidney. In conclusion, the immersion and dilution method is an adequate infection method for this disease. In order to achieve satisfactory infection, the precise management of water temperature and the dilution rate of bacterial culture are required.

収録刊行物

  • 水産増殖

    水産増殖 58 (4), 481-489, 2010

    日本水産増殖学会

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