In Vitro and In Vivo Genotoxicity Induced by Fullerene (C60) and Kaolin

• Totsuka Yukari

  Division of Cancer Development System, National Cancer Center Research Institute

• Kato Tatsuya

  Division of Cancer Development System, National Cancer Center Research Institute Department of Food & Nutritional Science, Graduate School of Nutrition & Environmental Science, University of Shizuoka

• Masuda Shu-ichi

  Department of Food & Nutritional Science, Graduate School of Nutrition & Environmental Science, University of Shizuoka

• Ishino Kousuke

  Division of Cancer Development System, National Cancer Center Research Institute

• Matsumoto Yoko

  Division of Cancer Development System, National Cancer Center Research Institute Laboratory of Environmental Risk Evaluation, School of Life and Enviromental Science, Azabu University

• Goto Sumio

  Laboratory of Environmental Risk Evaluation, School of Life and Enviromental Science, Azabu University

• Kawanishi Masanobu

  Environmental Genetics Laboratory, Frontier Science Innovation Center, Osaka Prefecture University

• Yagi Takashi

  Environmental Genetics Laboratory, Frontier Science Innovation Center, Osaka Prefecture University

• Wakabayashi Keiji

  Department of Food & Nutritional Science, Graduate School of Nutrition & Environmental Science, University of Shizuoka

抄録

Nanomaterials are being utilized for many kinds of industrial products, and the assessment of genotoxicity and safety of nanomaterials is therefore of concern. In the present study, we examined the genotoxic effects of fullerene (C₆₀) and kaolin using in vitro and in vivo genotoxicity systems. Both nanomaterials significantly induced micronuclei and enhanced frequency of sister chromatid exchange (SCE) in cultured mammalian cells. When ICR mice were intratracheally instilled with these nanomaterials, DNA damage of the lungs increased significantly that of the vehicle control. Formation of DNA adducts in the lungs of mice exposed to nanomaterials were also analyzed by stable isotope dilution LC-MS/MS. 8-Oxodeoxyguanosine and other lipid peroxide related adducts were increased by 2- to 5-fold in the nanomaterial-exposed mice. Moreover, multiple (four consecutive doses of 0.2 mg per animal per week) instillations of C₆₀ or kaolin, increased gpt mutant frequencies in the lungs of gpt delta transgenic mice. As the result of mutation spectrum analysis, G:C to C:G transversions were commonly increased in the lungs of mice exposed to both nanomaterials. In addition, G:C to A:T was increased in kaolin-exposed mice. In immunohistochemical analysis, many regions of the lungs that stained positively for nitrotyrosine (NT) were observed in mice exposed to nanomaterials. From these observations, it is suggested that oxidative stress and inflammatory responses are probably involved in the genotoxicity induced by C₆₀ and kaolin.<br>

収録刊行物

• Genes and Environment

  Genes and Environment 33 (1), 14-20, 2011

  日本環境変異原学会

キーワード

• nanomaterials
• genotoxicity
• fullerene (C₆₀)
• kaolin
• DNA adducts

詳細情報

• CRID: 1390001205257600512
• NII論文ID: 10027955671
• NII書誌ID: AA1212552X
• DOI: 10.3123/jemsge.33.14
• ISSN: 18807062
• ISSN: 18807046
• NDL書誌ID: 10981211
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10981211
• Web Site: https://search.jamas.or.jp/link/ui/2011336052
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可