放射性ヨウ化ナトリウム(131I)を注射したマウス血液のClearance-activityについて

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  • Clearance Test of the Blood of the Mouse Injected with Sodium Iodine (131I)
  • ホウシャセイ ヨウカ ナトリウム 131I オ チュウシャ シタ マウス ケツエキ ノ clearance-activity ニ ツイテ

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Experiments to bring about a diminution in the natural resistance of animal have already been made by workers.<br>The effect of sodium iodine on the innate immunity of mice was studied by the clearance of blood by the reticulo-endotherial system (RES). Mice were injected with sodium iodine (injection dose was 50μc per animal). After the injection, three intervals of time were setup. These were (a) a three-day interval after injection, (b) a 2-week interval after injection, and (c) a 5-week interval after injection. Nine mice were used respectively for each of above 3-time intervals, so 27 animals in all.<br>Next, all 9 mice of the 3-day interval were injected intravenously with 1mg of E. coli suspended in 0.1ml of NaCl solution. The E, coli had been cultivated for 24 hours in a incubator. Then 3 mice were selected from the nine, immediately after the injection of E, coli and the blood was taken from the heart by means of a syringe containing sodium citrate.A group of 3 other mice were similarly treated at a 30-minute interval after the injection of E. coli, and the remaining 3 mice at a 60-minute interval after the injection.<br>The amount of blood sample taken by the syrings was 0.5ml. Next, the liver, spleen and testes of each mouse were taken and emulsified. The concentration of these emulsions was adjusted to 100mg/ml, and the same organ emulsions of the 3 mice in each group were pooled respectively. Then, smples of the blood and of the liver, spleen and testes were diluted with NaCI solution, poured into Petri-dishes together with melted agar and cultivated for 48 hours in an incubator.<br>The bacterial colonies on the plates were counted, and the average number of bacteria in 1ml of blood was compared with that in the control animal. The similar procedures were repeated on the 9 mice in the 2 week and 5 week intervals. The results indicated that in the 9 mice injected with 50μc of sodium iodine and injected itravenously 3 days later with E. coli, the bacilli in the 30-minute animal were diminished and much more in the case of 60-minute animal. The clearance ability of sodium iodine injected mice was about the same as in the control mice. In those 9 mice which were injected with sodium iodine and injected intravenously 2 weeks later with E. coli, the 30 minute animals showed a decrease of bacilli in the blood stream and the 60-minute aniamis showed slightly more diminution. The untreated control mice showed a greater diminution of the bacilli (E. Coli) than the treated mice. When the mice were injected with E. coli 5 weeks after the sodium iodine injection, the "30-minute" and "60-minute" animals showed abundant numbers of bacilli in the blood stream in contrast to the diminution of the clearance ability in the control mice. Human leprous material was injected into the testes of these animals. The average body temperature during this week indicated a fall of about 1°C. The clearance ability of the liver, spleen and testes of the mice as tested, but it was not so significant as shown by figures of the blood stream.

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  • レプラ

    レプラ 40 (4), 194-198, 1971

    日本ハンセン病学会

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