Virology: One-step multiplex reverse-transcriptase PCR for detecting pandemic (H1N1) 2009 influenza virus

DOI Web Site Web Site 参考文献43件
  • SHIN Yeun-Kyung
    Virology Division, National Veterinary Research and Quarantine Service, Ministry for Food, Agriculture, Forestry and Fisheries
  • YOON Soon-Seek
    Virology Division, National Veterinary Research and Quarantine Service, Ministry for Food, Agriculture, Forestry and Fisheries
  • SONG Jae-Young
    Virology Division, National Veterinary Research and Quarantine Service, Ministry for Food, Agriculture, Forestry and Fisheries
  • KIM Joon-Bae
    Jeno Biotech Incorporation
  • HANG Do Thi Thu
    Virology Division, National Veterinary Research and Quarantine Service, Ministry for Food, Agriculture, Forestry and Fisheries
  • PARK Jin-Woo
    Virology Division, National Veterinary Research and Quarantine Service, Ministry for Food, Agriculture, Forestry and Fisheries
  • KIM Min-Young
    Virology Division, National Veterinary Research and Quarantine Service, Ministry for Food, Agriculture, Forestry and Fisheries
  • KIM Seong-Hee
    Disease Diagnostic Center, National Veterinary Research and Quarantine Service, Ministry for Food, Agriculture, Forestry and Fisheries
  • PARK Choi-Kyu
    Disease Diagnostic Center, National Veterinary Research and Quarantine Service, Ministry for Food, Agriculture, Forestry and Fisheries
  • LI O-Soo
    Disease Diagnostic Center, National Veterinary Research and Quarantine Service, Ministry for Food, Agriculture, Forestry and Fisheries

書誌事項

タイトル別名
  • One-Step Multiplex Reverse-Transcriptase PCR for Detecting Pandemic (H1N1) 2009 Influenza Virus

この論文をさがす

抄録

Pandemic (H1N1) 2009 influenza has spread throughout the world since April 2009 and has caused many human deaths since its first report in humans. Pandemic (H1N1) 2009 influenza virus was first identified in a Canadian pig herd in April 2009 and has been reported in more than ten countries, including Korea. We developed a one-step multiplex reverse transcriptase polymerase chain reaction (RT-PCR) assay based on the matrix gene that discriminates pandemic (H1N1) 2009 influenza virus from endemic swine influenza viruses. The sensitivity of this assay was 100 copies of in vitro-transcribed target RNA and 0.01 tissue culture infective dose (TCID50/ml) of virus and was as high as those of conventional influenza A virus common matrix reverse transcriptase PCR assays and real-time reverse transcriptase PCR assays (1 to 200 copies) developed for detecting pandemic (H1N1) 2009 influenza viruses from human and pig samples. This one-step multiplex RT-PCR assay would be a good tool in monitoring pandemic (H1N1) 2009 influenza virus among pig herds on a regular basis.<br>

収録刊行物

参考文献 (43)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ