Pentobarbital Decreased Nitric Oxide Release in the Rat Striatum but Ketamine Increased the Release Independent of Cholinergic Regulation

  • Kimura-Kuroiwa Kaori
    2nd Department of Anesthesia, Nagano Red Cross Hospital
  • Adachi Yushi U.
    Department of Emergency Medicine, Nagoya University Hospital
  • Mimuro Soichiro
    Department of Anesthesia and Resuscitation, Hamamatsu University School of Medicine
  • Kawamata Mikito
    Department of Anesthesiology and Resuscitology, Shinshu University School of Medicine
  • Sato Shigehito
    Department of Anesthesia and Resuscitation, Hamamatsu University School of Medicine
  • Matsuda Naoyuki
    Department of Emergency & Critical Care Medicine, Nagoya University Graduate School of Medicine

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抄録

Pentobarbital (PB) and ketamine (Ket) influence the concentration of neurotransmitters in the brain. PB has been reported to decrease the extracellular nitric oxide (NO) concentration through a decrease in acetylcholine (ACh) release, while Ket has been shown to increase the NO concentration via an increase in ACh release. Here, we investigated effects of PB and Ket on NO release and the relationship between NO and ACh in the rat striatum by in vivo microdialysis experiments. Male Sprague-Dawley rats were used. A microdialysis probe was inserted into the right striatum and perfused with modified Ringer’s solution. Samples were collected every 15 min and injected into an HPLC system. The rats were freely moving, and PB and Ket were administered intraperitoneally. Neostigmine (1 and 10 μM) and mecamylamine (100 μM) were added to the perfusate. Calcium and magnesium concentrations were modified for each anesthetic to influence ACh release. PB decreased NO products (NOx) while Ket increased them. While perfusion with neostigmine showed no effect on baseline NOx concentrations, it diminished the PB-induced NOx reduction at low concentrations and abolished it at high concentrations. Magnesium-free perfusion had no effect on baseline NOx concentrations, whereas perfusion at a low magnesium concentration antagonized the PB-induced NOx reduction. Mecamylamine and calcium-free perfusion had no effect on baseline NOx concentrations and Ket-induced NOx increases. PB may decrease NO release through reduction in ACh release, whereas Ket may increase NO release independent of ACh regulation.<br>

収録刊行物

  • Experimental Animals

    Experimental Animals 61 (2), 165-170, 2012

    公益社団法人 日本実験動物学会

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