Persistent C genome chromosome regions identified by SSR analysis in backcross progenies between Brassica juncea and B. napus

• Tsuda Mai

  Genetically Modified Organism Research Center, National Institute of Agrobiological Sciences (NIAS)

• Okuzaki Ayako

  Genetically Modified Organism Research Center, National Institute of Agrobiological Sciences (NIAS)

• Kaneko Yukio

  Laboratory of Plant Breeding, Faculty of Agriculture, Utsunomiya University

• Tabei Yutaka

  Genetically Modified Organism Research Center, National Institute of Agrobiological Sciences (NIAS)

書誌事項

タイトル別名

• Persistent C genome chromosome regions identified by SSR analysis in backcross progenies between <i>Brassica juncea</i> and <i>B. napus</i>

抄録

Given that feral transgenic canola (Brassica napus) from spilled seeds has been found outside of farmer’s fields and that B. juncea is distributed worldwide, it is possible that introgression to B. juncea from B. napus has occurred. To investigate such introgression, we characterized the persistence of B. napus C genome chromosome (C-chromosome) regions in backcross progenies by B. napus C-chromosome specific simple sequence repeat (SSR) markers. We produced backcross progenies from B. juncea and F₁ hybrid of B. juncea × B. napus to evaluate persistence of C-chromosome region, and screened 83 markers from a set of reported C-chromosome specific SSR markers. Eighty-five percent of the SSR markers were deleted in the BC₁ obtained from B. juncea × F₁ hybrid, and this BC₁ exhibited a plant type like that of B. juncea. Most markers were deleted in BC₂ and BC₃ plants, with only two markers persisting in the BC₃. These results indicate a small possibility of persistence of C-chromosome regions in our backcross progenies. Knowledge about the persistence of B. napus C-chromosome regions in backcross progenies may contribute to shed light on gene introgression.

収録刊行物

• Ｂｒｅｅｄｉｎｇ　Ｓｃｉｅｎｃｅ

  Ｂｒｅｅｄｉｎｇ　Ｓｃｉｅｎｃｅ 62 (4), 328-333, 2012

  日本育種学会

キーワード

• <i>Brassica napus</i>
• <i>Brassica juncea</i>
• introgression
• backcross progenies
• SSR marker
• transgenic canola
• C genome chromosome

詳細情報

• CRID: 1390001204723134336
• NII論文ID: 130004146347
• NII論文ID: 10031131501
• NII書誌ID: AA11317194
• DOI: 10.1270/jsbbs.62.328
• COI: 1:STN:280:DC%2BC3szitVCisA%3D%3D
• ISSN: 13473735
• ISSN: 13447610
• NDL書誌ID: 024151243
• PubMed: 23341746
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I024151243
• Web Site: https://www.jstage.jst.go.jp/article/jsbbs/62/4/62_328/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可