Efficient Synthesis of (+)-cis-12-Oxo-phytodienoic Acid by an in Vitro Enzymatic Reaction

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  • KAJIWARA Akiyuki
    Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University
  • ABE Tatsuya
    Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University
  • HASHIMOTO Takahiro
    Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University
  • MATSUURA Hideyuki
    Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University
  • TAKAHASHI Kosaku
    Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University

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  • Efficient Synthesis of (+)-<i>cis</i>-12-Oxo-phytodienoic Acid by an <i>in Vitro</i> Enzymatic Reaction

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12-Oxo-phytodienoic acid (OPDA) is an important metabolite on the octadecanoid pathway of plants. This study shows an efficient in vitro synthesis of (+)-cis-OPDA by using a flaxseed extract and an allene oxide cyclase. The OPDA yield of the reaction in this study was almost 7-fold higher than that in the conventional reaction with the flaxseed extract.

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