Toxoflavin Lyase Enzyme as a Marker for Selecting Potato Plant Transformants

• KIM Mi-Sun

  Green-Bio Research Center, KRIBB

• KIM HyunSoon

  Green-Bio Research Center, KRIBB

• MOON Jae Sun

  Green-Bio Research Center, KRIBB

• HWANG Ingyu

  Department of Agricultural Biotechnology and Center for Agricultural Biomaterials, Seoul National University

• JOUNG Hyouk

  Green-Bio Research Center, KRIBB

• JEON Jae-Heung

  Green-Bio Research Center, KRIBB

抄録

This study established a new system for potato transformation using toxoflavin as selection agent and toxoflavin lyase (tflA) as selectable marker gene. Potato plants expressing tflA was successfully transformed on toxoflavin medium with 27% efficiency, similar to that for the hygromycin/hpt selection system. The transgenic potato expressing tflA also showed resistance to Burkholderia glumea infection.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 76 (12), 2354-2356, 2012

  公益社団法人 日本農芸化学会

キーワード

• toxoflavin lyase (<i>tflA</i>)
• selection marker system
• light-dependence
• transformation efficiency

詳細情報

• CRID: 1390282681456644864
• NII論文ID: 130004137903
• NII論文ID: 10031146862
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.120593
• COI: 1:STN:280:DC%2BC3s7pvVSqtg%3D%3D
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 024166051
• PubMed: 23221711
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I024166051
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.120593
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可