Development of a promoter-luciferase-based high-throughput system to monitor jasmonate-mediated defense gene expression
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- Kusama Masahiro
- Graduate School of Environment and Information Sciences, Yokohama National University
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- Urata Nobuaki
- Graduate School of Environment and Information Sciences, Yokohama National University
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- Ogura Rieko
- Venture Business Laboratory, Yokohama National University
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- Ogata Shin-ichi
- Graduate School of Environment and Information Sciences, Yokohama National University
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- Hiratsuka Kazuyuki
- Graduate School of Environment and Information Sciences, Yokohama National University
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Defense gene expression systems of higher plants responsible for protection against pathogen attack are predominantly regulated by salicylic acid (SA)- and jasmonic acid (JA)-mediated pathways, and control the expression of many downstream defense response genes. To monitor the regulated gene expression of SA-mediated signaling pathways, we previously described an assay system based on the bioluminescence of seedlings transformed with a promoter-luciferase fusion gene. Here, to develop a system suitable for JA-mediated gene expression monitoring, we compared the expression patterns of Arabidopsis gene promoters obtained from the plant defensin 1.2 (PDF1.2) and vegetative storage protein 1 (VSP1) genes in response to treatment with chemicals. Although both promoters responded well to treatment with JA in 3-week-old plants, only the VSP1 promoter exhibited marked and prolonged luciferase expression in response to JA treatment in 6-day-old seedlings. The use of transgenic Arabidopsis seedlings harboring the VSP1-luciferase reporter gene construct enables multiwell plates to be used for conducting high-throughput assays for the screening of chemicals that are involved in JA-mediated signaling pathways in Arabidopsis.
収録刊行物
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- Plant Biotechnology
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Plant Biotechnology 29 (5), 515-520, 2012
日本植物バイオテクノロジー学会
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詳細情報 詳細情報について
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- CRID
- 1390282679305856128
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- NII論文ID
- 130002500895
- 40019545410
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- NII書誌ID
- AA11250821
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- ISSN
- 13476114
- 13424580
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- NDL書誌ID
- 024195726
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- 使用不可