ヒラメのウイルス性出血性敗血症に対するホルマリン不活化ワクチンの感染防御効果

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タイトル別名
  • Protective Efficacy of a Formalin-inactivated Vaccine against Viral Hemorrhagic Septicemia in Japanese Flounder <i>Paralichthys olivaceus</i>
  • Protective Efficacy of a Formalin-inactivated Vaccine against Viral Hemorrhagic Septicemia in Japanese Flounder Paralichthys olivaceus

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We examined the effectiveness of a formalin-inactivated vaccine against viral hemorrhagic septicemia (VHS) in Japanese flounder Paralichthys olivaceus by the pathogen challenge. Japanese flounder (average weight, 10 g) kept at 12°C, 20°C and 28°C were vaccinated by intraperitoneal injection of a formalin-inactivated VHS virus (VHSV) and maintained at each temperature for 21, 19 and 17 days post vaccination (dpv), respectively. The fish vaccinated at 20°C and 28°C were acclimated to 12°C, and then all vaccinated fish were challenged with VHSV at 12°C at 21 dpv. Fish that received the vaccine at 20°C showed relative percent survival (RPS) of 48%, compared with 0% for those given the vaccine at 12°C and 28°C. In other experiment, efficient protection against VHSV lasted at least 7 mo in Japanese flounder (average weight, 100 g) vaccinated at 20°C and maintained at ambient temperature, where high RPS values (71-100%) were obtained after viral challenges at 18, 77, 98 and 213 dpv. We obtained promising results that a single immunization with the formalin-inactivated vaccine at the physiologically optimal temperature (20°C) provided significant protective and long-lasting immunity to Japanese flounder against VHS.

収録刊行物

  • 魚病研究

    魚病研究 47 (4), 121-128, 2012

    日本魚病学会

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