4′-チオDNAを用いたRNA創薬

• 南川 典昭

  徳島大学大学院ヘルスバイオサイエンス研究部

書誌事項

タイトル別名

• Development of RNA Medicine Using 4′-thioDNA
• 4'-チオDNAを用いたRNA創薬
• 4'-チオ DNA オ モチイタ RNAソウヤク

抄録

  A number of modified oligonucleotides prepared using a general chemical approach with the corresponding phosphoramidite units have been synthesized to evaluate their functions. An alternative enzymatic method using the corresponding nucleoside triphosphates could also be used. Since this approach affords long-chain sequences from readily available natural DNA templates, if successful it would be useful in numerous biotechnologies. This review summarizes our current results of polymerase chain reaction (PCR) amplification of 4′-thioDNA using 4′-thio-dNTPs and gene silencing using the resulting 4′-thioDNAs as a template arising from 4′-thioDNA-directed transcription in mammalian cells.<br>

収録刊行物

• 薬学雑誌

  薬学雑誌 133 (1), 53-60, 2013-01-01

  公益社団法人 日本薬学会

キーワード

• 4′-thioDNA
• polymerase chain reaction (PCR)
• RNA interference
• short-hairpin RNA

詳細情報

• CRID: 1390001206127417728
• NII論文ID: 130003361879
• NII書誌ID: AN00284903
• DOI: 10.1248/yakushi.12-00245-2
• COI: 1:STN:280:DC%2BC3s3ntVegug%3D%3D
• ISSN: 13475231
• ISSN: 00316903
• NDL書誌ID: 024214169
• PubMed: 23292020
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I024214169
• Web Site: https://www.jstage.jst.go.jp/article/yakushi/133/1/133_12-00245-2/_pdf
• 本文言語コード: ja
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