Structural Characteristics and Evolution of the Protobothrops elegans Pancreatic Phospholipase A₂ Gene in Contrast with Those of Protobothrops Genus Venom Phospholipase A₂ Genes

• CHIJIWA Takahito

  Department of Applied Life Science, Faculty of Bioscience and Biotechnology, Sojo University

• NAKASONE Hideto

  Department of Applied Life Science, Faculty of Bioscience and Biotechnology, Sojo University

• IRIE Sakiko

  Department of Applied Life Science, Faculty of Bioscience and Biotechnology, Sojo University

• IKEDA Naoki

  Department of Applied Life Science, Faculty of Bioscience and Biotechnology, Sojo University

• TOMODA Kantaro

  Department of Applied Life Science, Faculty of Bioscience and Biotechnology, Sojo University

• ODA-UEDA Naoko

  Department of Biochemistry, Faculty of Pharmaceutical Sciences, Sojo University

• HATTORI Shosaku

  Institute of Medical Science, University of Tokyo

• OHNO Motonori

  Department of Applied Life Science, Faculty of Bioscience and Biotechnology, Sojo University

書誌事項

タイトル別名

• Structural Characteristics and Evolution of the <i>Protobothrops elegans</i> Pancreatic Phospholipase A₂ Gene in Contrast with Those of <i>Protobothrops</i> Genus Venom Phospholipase A₂ Genes

抄録

The nucleotide sequence of the gene encoding Protobothrops elegans (Crotalinae) pancreatic phospholipase A₂ (PLA₂), abbreviated PePancPLA₂, was determined by means of inverted PCR techniques. Since its deduced amino acid sequence contains a pancreatic loop and shows high similarity to that of Laticauda semifasciata (Elapinae) group IB pancreatic PLA₂, PePancPLA₂ is classified into group IB PLA₂. The nucleotide sequences of the PePancPLA₂ gene, the L. semifasciata group IB pancreatic PLA₂ gene, and the L. semifasciata group IA venom PLA₂ gene are similar to one another but greatly dissimilar to those of Protobothrops genus (Crotalinae) group II venom PLA₂ genes, suggesting that the Elapinae group IB PLA₂ gene and the group IA PLA₂ gene appeared after Elapinae was established, and that the Crotalinae group II venom PLA₂ genes came into existence before Elapinae and Crotalinae diverged. A phylogenetic analysis of their amino acid sequences confirms this.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 77 (1), 97-102, 2013

  公益社団法人 日本農芸化学会

キーワード

• amino acid sequence
• evolution
• gene
• pancreatic phospholipase A₂
• <i>Protobothrops elegans</i>

詳細情報

• CRID: 1390001206478610816
• NII論文ID: 10031164697
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.120595
• COI: 1:STN:280:DC%2BC3s3ntVSruw%3D%3D
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 024226536
• PubMed: 23291767
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I024226536
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.120595
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可