Application of LDH-Release Assay to Cellular-Level Evaluation of the Toxic Potential of Harmful Algal Species

• ZOU Yanan

  Division of Biochemistry, Faculty of Fisheries, Nagasaki University

• KIM Daekyung

  Jeju Center, Korea Basic Science Institute (KBSI)

• YAGI Motoaki

  Joint Research Division, Center for Industry, University and Government Cooperation, Nagasaki University

• YAMASAKI Yasuhiro

  Laboratory of Environmental Biology, Department of Applied Aquabiology, National Fisheries University

• KURITA Jun

  National Research Institute of Aquaculture, Fisheries Research Agency

• IIDA Takaji

  National Research Institute of Aquaculture, Fisheries Research Agency

• MATSUYAMA Yukihiko

  Seikai National Fisheries Research Institute

• YAMAGUCHI Kenichi

  Division of Biochemistry, Faculty of Fisheries, Nagasaki University

• ODA Tatsuya

  Division of Biochemistry, Faculty of Fisheries, Nagasaki University

抄録

Lactate dehydrogenase (LDH)-release assay was applied to estimate the toxic potential of harmful algal species at the cellular level. African green monkey kidney (Vero), yellowtail fin epithelia (MJF), and rainbow trout gill (RTgill-W1) cells were used as target cells. A live cell suspension of Karenia mikimotoi (SUO-1) induced the release of LDH from these cell lines, while the activity of another strain, FUK, was much lower. The cell-free culture supernatants and ruptured cell suspensions of both strains of K. mikimotoi were less effective on LDH-release assay. Exposure experiments against abalone and shrimp revealed that SUO-1 showed much stronger lethal effects on these organisms than FUK. Among six phytoplankton species, three species known to be harmful algal species induced the release of LDH to different extents depending on the cell line, whereas the other three species, known to be non-toxic, showed no effects on any cell lines. These results suggest that LDH-release assay is a useful micro-plate assay for estimation of the toxic potential of harmful phytoplankton.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 77 (2), 345-352, 2013

  公益社団法人 日本農芸化学会

キーワード

• lactate dehydrogenase (LDH)-release assay
• <i>Karenia mikimotoi</i>
• harmuful algal blooms (HABs)
• African green monkey kidney (Vero) cells
• cell culture

詳細情報

• CRID: 1390282681453927296
• NII論文ID: 10031164739
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.120764
• COI: 1:STN:280:DC%2BC3szmsFKrtg%3D%3D
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 024295484
• PubMed: 23391929
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I024295484
• Web Site: https://search.jamas.or.jp/link/ui/2013276372
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可