Isolation and Identification of 4,4'-Diapolycopene-4,4'-dioic Acid Produced by Bacillus firmus GB1 and its Singlet Oxygen Quenching Activity
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- Osawa Ayako
- Department of Food and Nutrition, Japan Women’s University
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- Iki Kanoko
- Department of Food and Nutrition, Japan Women’s University
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- Sandmann Gerhard
- Biosynthesis Group, Institute of Molecular Biosciences, Goethe University
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- Shindo Kazutoshi
- Department of Food and Nutrition, Japan Women’s University
書誌事項
- タイトル別名
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- Isolation and Identification of 4, 4’-Diapolycopene-4,4’-dioic Acid Produced by <i>Bacillus firmus</i> GB1 and its Singlet Oxygen Quenching Activity
- Isolation and Identification of 4, 4^|^rsquo;-Diapolycopene-4,4^|^rsquo;-dioic Acid Produced by Bacillus firmus GB1 and its Singlet Oxygen Quenching Activity
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We succeeded in purifying the carotenoid produced by Bacillus firmus GB1 using chromatography on polystyrenic synthetic adsorbents, and identified it as 4,4’-diapolycopene-4,4’-dioic acid by HRESI-MS and NMR analyses of it and its dimethylester. In addition, we clarified the singlet oxygen (1O2) quenching activities of 4,4’-diapolycopene-4,4’-dioic acid and its methyl esters (mono and dimethyl ester). Their IC50 values were 5.8 μM, 6.0 μM, and 6.2 μM, respectively. To our knowledge, this is the first report concerning the isolation and antioxidative activity of 4,4’-diapolycopene-4,4’-dioic acid, a product of the C30 carotenoid biosynthesis pathway.
収録刊行物
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- Journal of Oleo Science
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Journal of Oleo Science 62 (11), 955-960, 2013
公益社団法人 日本油化学会
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詳細情報 詳細情報について
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- CRID
- 1390282679068894720
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- NII論文ID
- 130004436007
- 40019836691
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- NII書誌ID
- AA11503337
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- COI
- 1:STN:280:DC%2BC2c7jvFWisw%3D%3D
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- ISSN
- 13473352
- 13458957
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- NDL書誌ID
- 024947617
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- PubMed
- 24200944
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 使用不可