Quantitative Determination of Cyclosporine in Human Whole Blood by Ultra-Performance Liquid Chromatography with Triple Quadrupole Tandem Mass Spectrometry

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  • JUNG Hyun Jin
    Department of Biomedical Science and Clinical Trial Center, Kyungpook National University Graduate School and Hospital
  • GWON Mi-Ri
    Department of Biomedical Science and Clinical Trial Center, Kyungpook National University Graduate School and Hospital KNU Bio-Medical Convergence Program for Creative Talent, Kyungpook National University Graduate School
  • PARK Jeonghyeon
    Department of Biomedical Science and Clinical Trial Center, Kyungpook National University Graduate School and Hospital
  • SEO Jeong Ju
    Department of Biomedical Science and Clinical Trial Center, Kyungpook National University Graduate School and Hospital
  • SEONG Sook Jin
    Department of Biomedical Science and Clinical Trial Center, Kyungpook National University Graduate School and Hospital
  • KIM Eun Hee
    School of Nursing, Yeungnam College of Science & Technology
  • SUH Soon Rim
    College of Nursing, Kyungpook National University
  • JEONG Ji Yun
    Division of Endocrinology and Metabolism, Department of Internal Medicine, Kyungpook National University School of Medicine
  • LEE Hae Won
    Department of Biomedical Science and Clinical Trial Center, Kyungpook National University Graduate School and Hospital
  • YOON Young-Ran
    Department of Biomedical Science and Clinical Trial Center, Kyungpook National University Graduate School and Hospital KNU Bio-Medical Convergence Program for Creative Talent, Kyungpook National University Graduate School

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Cyclosporine is an immunosuppressant drug used in organ transplants or for the treatment of autoimmune diseases. We developed and validated a simple, sensitive, and specific method using UPLC-MS/MS to determine cyclosporine levels in human whole blood. MS/MS detection was performed in the positive electrospray ionization mode with multiple reaction monitoring. Cyclosporine was extracted from whole-blood samples using ascomycin as an internal standard. The mass transitions m/z 1203.49 → 1185.53 and m/z 814.71 → 796.67 were used to assay the analyte and IS. This method was validated with respect to linearity, specificity, accuracy, precision, recovery, and stability. The method exhibited a linear response from 10 to 1000 ng mL−1 with correlation coefficient values >0.99. The precision and the accuracy values were within 15%, except at the lower limit of quatification (LLOQ). Cyclosporine was stable in whole blood with no evidence of degradation. This method was successfully applied to a pharmacokinetic study of cyclosporine in healthy volunteers following oral administration.

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  • Analytical Sciences

    Analytical Sciences 30 (2), 293-298, 2014

    社団法人 日本分析化学会

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