<i>In vitro</i> evaluation of dehydration tolerance in <i>AtDREB1A</i> transgenic potatoes

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  • Huynh Huu Duc
    Graduate School of Life and Environmental Sciences, University of Tsukuba
  • Shimazaki Takayoshi
    Gene Research Center, Faculty of Life and Environmental Sciences, University of Tsukuba
  • Kasuga Mie
    Biological Resources Division, Japan International Research Center for Agricultural Sciences (JIRCAS)
  • Yamaguchi-Shinozaki Kazuko
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • Kikuchi Akira
    Graduate School of Life and Environmental Sciences, University of Tsukuba Gene Research Center, Faculty of Life and Environmental Sciences, University of Tsukuba
  • Watanabe Kazuo N.
    Graduate School of Life and Environmental Sciences, University of Tsukuba Gene Research Center, Faculty of Life and Environmental Sciences, University of Tsukuba

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  • In vitro evaluation of dehydration tolerance in AtDREB1A transgenic potatoes

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Abstract

Abiotic stresses have negative effects on potato growth and production. To enhance the abiotic stress tolerance of the commercial potato cultivar, Desiree, rd29A::AtDREB1A transgenic lines have been developed. The salinity and freeze tolerance of these transgenic lines has previously been demonstrated; however, their dehydration tolerance remains to be elucidated. First of all, we have tackled the reproducible tolerance evaluation methodology, which have been the hurdle for selecting the stable dehydration tolerant phenotypes in potato. A novel in vitro method was developed using the rotary liquid culture combined with PEG. This method enhanced oxygen diffusion into the medium and diminished root damage/injury during plant transfer, thereby reducing the side effects caused by hypoxia and penetration of osmotica. In the present study, we evaluated the dehydration tolerance of twelve transgenic potato lines, and seven of the transgenic lines showed enhanced dehydration tolerance in comparison with the non-transgenic line. However, we observed growth retardation in some dehydration tolerant lines. Therefore, balance between the dehydration tolerance and growth retardation of the transgenic plants should be considered. Four of seven of the transgenic lines displayed enhanced dehydration tolerance without growth retardation, and may represent good candidates for practical application.

Journal

  • Plant Biotechnology

    Plant Biotechnology 31 (1), 77-81, 2014

    Japanese Society for Plant Biotechnology

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