単一細胞操作技術を用いたB16メラノーマ細胞の選別と単一細胞における遺伝子配列解析

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  • Selection of the B16 melanoma cells with use of single cell manipulation technique and sequence analysis of the DNA in the single cell
  • タンイツ サイボウ ソウサ ギジュツ オ モチイタ B16 メラノーマ サイボウ ノ センベツ ト タンイツ サイボウ ニ オケル イデンシ ハイレツ カイセキ

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Epigenetics is study about regulation of gene expression without changing DNA sequence. This means that difference in gene expression occur between cells due to modification state of promoter region in this gene if those have same gene. Melanin which is known as the cause of the stain is produced when tyrosine was oxidized by tyrosinase. Melanin is remains on the skin after the turnover of the skin cells. In our laboratory, we observed that tyrosinase may be affected by an epigenetics in melanin production. In this study, we investigated basic experiment of epigenetic analysis by using Pico-Pipet which capable of single cell manipulation. Single cell was selected by Pico-Pipet after cultivation of B16 melanoma cells. Expression of tyrosinase in extracted DNA from the single melanoma cell was evaluated. And the sequence of tyrosinase gene was analyzed. In the results, the expression of tyrosinase gene in a single cell was confirmed. Analysis sequence of tyrosinase gene was compared with database and the concordance rate of the sequence of tyrosinase was 100 %.

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