Circadian Variation of Rho-Kinase Activity in Circulating Leukocytes of Patients With Vasospastic Angina

• Nihei Taro

  Department of Cardiovascular Medicine, Tohoku University Graduate School of Medicine

• Takahashi Jun

  Department of Cardiovascular Medicine, Tohoku University Graduate School of Medicine

• Tsuburaya Ryuji

  Department of Cardiovascular Medicine, Tohoku University Graduate School of Medicine

• Ito Yoshitaka

  Department of Cardiovascular Medicine, Tohoku University Graduate School of Medicine

• Shiroto Takashi

  Department of Cardiovascular Medicine, Tohoku University Graduate School of Medicine

• Hao Kiyotaka

  Department of Cardiovascular Medicine, Tohoku University Graduate School of Medicine

• Takagi Yusuke

  Department of Cardiovascular Medicine, Tohoku University Graduate School of Medicine

• Matsumoto Yasuharu

  Department of Cardiovascular Medicine, Tohoku University Graduate School of Medicine

• Nakayama Masaharu

  Department of Cardiovascular Medicine, Tohoku University Graduate School of Medicine

• Miyata Satoshi

  Department of Cardiovascular Medicine, Tohoku University Graduate School of Medicine

• Sakata Yasuhiko

  Department of Cardiovascular Medicine, Tohoku University Graduate School of Medicine

• Ito Kenta

  Department of Cardiovascular Medicine, Tohoku University Graduate School of Medicine

• Shimokawa Hiroaki

  Department of Cardiovascular Medicine, Tohoku University Graduate School of Medicine

抄録

Background: Vasospastic angina (VSA) is known to exhibit circadian variation with an early morning peak. We examined whether Rho-kinase activity in circulating leukocytes, which is a useful biomarker for disease activity assessment of VSA, exhibits circadian variation in patients with VSA. Methods and Results: In consecutive 31 VSA patients (M/F 23/8, 57±13 [SD] years) and 18 non-VSA patients (M/F 8/10, 57±14 years), we measured Rho-kinase activity in circulating leukocytes at 6:00, 12:00 and 21:00. We also examined the relationship between the Rho-kinase activity and coronary vasomotor responses during provocation test. Rho-kinase activity was significantly higher in VSA patients than in non-VSA patients at 6:00 (1.17±0.17 vs. 0.92±0.22, P<0.001), and showed a significant circadian variation with a peak at 6:00 (1.00±0.15 at 21:00, 1.17±0.17 at 6:00 and 1.12±0.22 at 12:00, P<0.001) in VSA patients, whereas no such variation was noted in non-VSA patients. Importantly, Rho-kinase activity at spasm provocation test was significantly correlated with basal coronary tone defined by vasodilating responses to intracoronary nitrate (r=0.40, P<0.05) and coronary vasoconstricting responses to acetylcholine (r=0.44, P<0.05) in VSA patients. Furthermore, their Rho-kinase activity at 6:00 was positively correlated with nocturnal parasympathetic activity as evaluated by heart rate variability in Holter monitoring (r=0.48, P<0.05). Conclusions: Rho-kinase activity exhibits distinct circadian variation associated with alterations in coronary vasomotor responses and autonomic activity in VSA patients.  (Circ J 2014; 78: 1183–1190)<br>

収録刊行物

• Circulation Journal

  Circulation Journal 78 (5), 1183-1190, 2014

  一般社団法人 日本循環器学会

キーワード

• Circadian variation
• Rho-kinase activity
• Vasospastic angina

詳細情報

• CRID: 1390282680084032000
• NII論文ID: 130003391088
• NII書誌ID: AA11591968
• DOI: 10.1253/circj.cj-13-1458
• COI: 1:STN:280:DC%2BC2crnvVSkuw%3D%3D
• ISSN: 13474820
• ISSN: 13469843
• ISSN: http://id.crossref.org/issn/13469843
• NDL書誌ID: 025412883
• PubMed: 24670923
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I025412883
• Web Site: https://www.jstage.jst.go.jp/article/circj/78/5/78_CJ-13-1458/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2015113384
• 本文言語コード: en
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