Ultrastructural changes of cytochemical Mg<sup>2+</sup>-ATPase on three muscle types of cultured carp (<i>Cyprinus carpio</i>) during storage in ice

  • Liang Jia
    Laboratory of Food Nutritional Science, Graduate School of Fisheries Sciences & Environmental Studies, Nagasaki University
  • Miyazaki Riho
    Laboratory of Food Nutritional Science, Graduate School of Fisheries Sciences & Environmental Studies, Nagasaki University
  • Zhao Xianxian
    Laboratory of Food Nutritional Science, Graduate School of Fisheries Sciences & Environmental Studies, Nagasaki University
  • Yoshida Asami
    Laboratory of Biorogical Chemistry, Graduate School of Fisheries Sciences & Environmental Studies, Nagasaki University
  • Hirasaka Katsuya
    Laboratory of Food Nutritional Science, Graduate School of Fisheries Sciences & Environmental Studies, Nagasaki University
  • Taniyama Shigeto
    Laboratory of Food Nutritional Science, Graduate School of Fisheries Sciences & Environmental Studies, Nagasaki University
  • Tachibana Katsuyasu
    Laboratory of Food Nutritional Science, Graduate School of Fisheries Sciences & Environmental Studies, Nagasaki University

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Other Title
  • コイの筋タイプ別細胞化学的Mg<sup>2+</sup>-ATPase活性の氷蔵中における変化
  • コイの筋タイプ別細胞化学的Mg²⁺-ATPase活性の氷蔵中における変化
  • コイ ノ キン タイプ ベツ サイボウ カガクテキ Mg ² ⁺-ATPase カッセイ ノ コオリグラ チュウ ニ オケル ヘンカ

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Abstract

We performed the comparative study on changes of cytochemical Mg2+-adenosine triphosphatase (Mg2+-ATPase) activity and its localization caused by a decrease in freshness of white (W), pink (P), and red (R) muscles in cultured carp during storage in ice. The contents of ATP-related compounds in the three muscle types were higher in the order of R>P>W, and the increasing of K value was faster in the order of R>P>W. Mg2+-ATPase activity was found on the A band of myofibril, sarcoplasmic reticulum and mitochondria menbrane/crista in each muscle type at just after killing. The deactivation of Mg2+-ATPase activity in these organella was faster in the order of R>P>W during storage period. The interposition of pink muscle fibers into the dorsal ordinary muscle might accelerate the deterioration of fish mucle in post-mortem.

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