Electrochemistry of Eu(III) Binding to N-terminal of Euplotes Octocarinatus Centrin

• RONG Zhijiang

  Institute of Molecular Science, Key Laboratory of Chemical Biology of Molecular Engineering of Education Ministry, Shanxi University School of Environment and Safety, Taiyuan University of Science and Technology

• MA Ling

  College of Chemistry & Chemical Engineering, Jinzhong University

• LIU Bin

  Institute of Molecular Science, Key Laboratory of Chemical Biology of Molecular Engineering of Education Ministry, Shanxi University

• TIAN Yanni

  Institute of Molecular Science, Key Laboratory of Chemical Biology of Molecular Engineering of Education Ministry, Shanxi University

• YANG Binsheng

  Institute of Molecular Science, Key Laboratory of Chemical Biology of Molecular Engineering of Education Ministry, Shanxi University

抄録

Ciliate Euplotes octocarinatus centrin (EoCen) is a member of the EF-hand super family of calcium-binding proteins, which is associated with the centrosomes. In this work, the interaction between N-terminal of EoCen (N-EoCen) and Eu³⁺ was investigated by cyclic voltammetry and UV-Vis spectroscopies. The result shows that a 2:1 of Eu³⁺ to N-EoCen complex (Eu₂-N-EoCen) is formed. The electrochemical characteristics of Eu₂-N-EoCen at a multi-wall carbon nanotube/Nafion composite film modified glassy carbon electrode (MWNT/Nafion/GCE) were then investigated in detail, from which a direct, quasi reversible electron transfer of Eu₂-N-EoCen had been obtained. According to the Laviron diffusionless-controled equations, the electron transfer rate constant k_s, was calculated to be 2.33 ± 0.14 s⁻¹. The pH dependence of the formal potential of Eu₂-N-EoCen suggests that protonation accompanies electron transfer between pH 5.5–7.4. The electrochemical redox and quantitative analysis of Eu₂-N-EoCen are therefore of great significance not only studying the physiological processes of Eu₂-N-EoCen in organisms but also the interaction mechanism with target protein.

収録刊行物

• Electrochemistry

  Electrochemistry 82 (8), 663-669, 2014

  公益社団法人　電気化学会

キーワード

• Eu³⁺
• Centrin
• Electrochemical Parameters
• Carbon Nanotube

詳細情報

• CRID: 1390001206498936448
• NII論文ID: 130004680080
• NII論文ID: 40020143464
• NII書誌ID: AN00151637
• DOI: 10.5796/electrochemistry.82.663
• ISSN: 21862451
• ISSN: 13443542
• NDL書誌ID: 025621931
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I025621931
• Web Site: https://www.jstage.jst.go.jp/article/electrochemistry/82/8/82_14-E00007/_pdf
• 本文言語コード: en
• データソース種別:
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• 抄録ライセンスフラグ: 使用可