Cytoprotective Effect of Kaempferol on Paraquat-Exposed BEAS-2B Cells via Modulating Expression of MUC5AC
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- Podder Biswajit
- Department of Microbiology, College of Medicine, Soonchunhyang University
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- Song Kyoung Seob
- Department of Physiology, Kosin University College of Medicine
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- Song Ho-Yeon
- Department of Microbiology, College of Medicine, Soonchunhyang University
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- Kim Yong-Sik
- Department of Microbiology, College of Medicine, Soonchunhyang University
書誌事項
- タイトル別名
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- Cytoprotective Effect of Kaempferol on Paraquat-Exposed BEAS-2B Cells <i>via</i> Modulating Expression of <i>MUC5AC</i>
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Mucins are highly glycosylated secretary proteins produced by most epithelial cells. Hypersecretion of mucins is one of the prominent symptoms of several airway diseases, including asthma, cystic fibrosis, nasal allergy, rhinitis, and sinusitis. Paraquat (PQ), a common herbicide, has been associated with pulmonary damage and is a potent reactive oxygen species (ROS) producer. However, until now the role of PQ on mucin overproduction has not been studied. The aim of this study is to explore how kaempferol (KM), a widely used dietary flavonoid, affects the protection of human PQ-exposed bronchial epithelium BEAS-2B cells by suppressing Mucin gene expression via nuclear factor-kappa B (NF-κB). We observed that PQ generates intracellular ROS, and also induces lipid peroxidation in BEAS-2B cells. Additionally, we found that PQ effectively induces the expression of the MUC5AC gene; however, co-treatment of PQ with KM drastically reduces its expression. Furthermore, we observed that PQ activates NF-κB, while co-treatment with KM occludes its nuclear translocation, and additionally KM repressed the PQ phosphorylation of c-Jun N-terminal kinase (JNK) and extracellular signal-regulated kinase (ERK) in BEAS-2B cells. Based on our data, we believe that KM can suppress the over-expression of the MUC5AC gene. This would contribute to the protection of PQ cytotoxicity to exposed BEAS-2B cells, and allow further study toward a better understanding of ROS-associated diseases.
収録刊行物
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- Biological & Pharmaceutical Bulletin
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Biological & Pharmaceutical Bulletin 37 (9), 1486-1494, 2014
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390001204633728768
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- NII論文ID
- 130004684789
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- NII書誌ID
- AA10885497
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- COI
- 1:STN:280:DC%2BC2M%2FmvFKhug%3D%3D
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- ISSN
- 13475215
- 09186158
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- NDL書誌ID
- 025736917
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- PubMed
- 25177032
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可