Application of 5-aminolevulinic Acid for the in vitro Micropropagation of Cymbidium as a Potential Novel Plant Regulator
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- NAHAR Syeda Jabun
- The United Graduate School of Agricultural Sciences, Ehime University
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- SHIMASAKI Kazuhiko
- Faculty of Agriculture, Kochi University
書誌事項
- タイトル別名
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- Application of 5-aminolevulinic Acid for the <i>in vitro</i> Micropropagation of <i>Cymbidium</i> as a Potential Novel Plant Regulator
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In this study we investigated the effect of 5-aminolevulinic acid (ALA) on the in vitro regulation of protocorm-like bodies (PLBs) of Cymbidium spp. PLBs of Cymbidium species were explanted on modified Murashige and Skoog (MS) medium supplemented with various concentrations of ALA. In Cymbidium insigne, 1 mg L−1 ALA induced the highest PLB (100%) proliferation rate with an average of 7.9 PLBs/explant, whereas the control treatment had 1.6 PLBs/explant. The highest shoot formation rate (66.7%) and the highest number of shoots (1.3 shoots/explant) were observed at 0.1 mg L−1 ALA. In Cymbidium finlaysonianum, the highest PLB formation rate (100%) and the highest average number of PLBs of (7.4 PLBs/explant) were observed for explants cultured on medium supplemented with 0.01 mg L−1 ALA. The highest number of shoots (0.7 shoots/explants) with a shoot proliferation rate of 33% was observed on medium supplemented with 0.1 mg L−1 ALA. The results of this study suggest that a low concentration of ALA can induce PLB and shoot formation and that ALA can serve as a novel additive for plant tissue culture acting as a growth regulator for Cymbidium micropropagation.
収録刊行物
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- Environment Control in Biology
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Environment Control in Biology 52 (3), 117-121, 2014
日本生物環境工学会
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詳細情報 詳細情報について
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- CRID
- 1390282680235563264
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- NII論文ID
- 130004691357
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- NII書誌ID
- AA12029220
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- ISSN
- 18830986
- 1880554X
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- NDL書誌ID
- 025768430
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可