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- Takahashi Saori
- Akita Research Institute of Food and Brewing (ARIF)
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- Gotoh Takeshi
- Department of Applied Chemistry, Akita University
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- Hata Keishi
- Akita Research Institute of Food and Brewing (ARIF)
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- Tokiwano Tetsuo
- Fuclty of Bioresource Science, Akita Prefectural University
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- Yoshizawa Yuko
- Fuclty of Bioresource Science, Akita Prefectural University
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- Hiwatashi Kazuyuki
- Akita Research Institute of Food and Brewing (ARIF)
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- Ogasawara Hironobu
- Akita Research Institute of Food and Brewing (ARIF)
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- Hori Kazuyuki
- Akita Research Institute of Food and Brewing (ARIF)
この論文をさがす
抄録
Renin, a highly specific aspartic proteinase originated from kidney, is a ratelimiting enzyme in renin-angiotensin system. Recently, we developed effective expression system for recombinant human (rh) renin using baculovirus-insect cell expression system, and simple and rapid assay method for rh-renin using internally quenched fluorogenic (IQF) substrate. Using the purified rh-renin and the IQF substrate, we constructed rapid and sensitive renin inhibitor screening system. Using the system, we found renin inhibitors in various foodstuffs. The isolation and characterization of renin inhibitors from soybean, rice, and wild vegetables and structure-function relationship of the inhibitors are also discussed.
収録刊行物
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- Journal of Biological Macromolecules
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Journal of Biological Macromolecules 14 (2), 71-84, 2014
日本生物高分子学会