Anti-adipogenic Constituents from Dioscorea opposita in 3T3-L1 Cells
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- Yang Min Hye
- College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University
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- Chin Young-Won
- College of Pharmacy, Dongguk University–Seoul
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- Chae Hee-Sung
- College of Pharmacy, Dongguk University–Seoul
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- Yoon Kee Dong
- College of Pharmacy, The Catholic University of Korea
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- Kim Jinwoong
- College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University
書誌事項
- タイトル別名
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- Anti-adipogenic Constituents from <i>Dioscorea opposita</i> in 3T3-L1 Cells
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抄録
We previously reported the lipase inhibitory activity of the n-BuOH fraction of Dioscorea opposita (DOB) and its isolates. This study sought to evaluate their anti-adipogenic activity in terms of their effects on the adipogenic transcription factors peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT/enhancer binding protein α (C/EBPα) as well as phosphorylated AMP-activated protein kinase (p-AMPK) and carnitine palmitoyl transferase-1 (CPT-1). DOB apparently attenuated 3T3-L1 adipocyte differentiation (33.6% decrease at 20 µg/mL). In addition, a marked decrease (90.4%) in the expression of PPARγ was observed in the DOB-treated 3T3-L1 cells. Four isolates from DOB: (4E,6E)-1,7-bis(4-hydroxyphenyl)-4,6-heptadien-3-one (1), (3R,5R)-1,7-bis(4-hydroxy-3-methoxyphenyl)-3,5-heptanediol (2), batatasin I (3), and (1E,4E,6E)-1,7-bis(4-hydroxyphenyl)-1,4,6-heptatrien-3-one (4), suppressed adipocyte differentiation by inhibiting PPARγ at 20 µM (85.9%, 68.6%, 76.2%, and 90.2% decrease, respectively) and C/EBPα (51.7%, 3.1%, 20.9%, and 59.8% decrease, respectively). Batatasin I was found to increase p-AMPK and CPT-1 at a concentration of 20 µM in 3T3-L1 adipocytes, resulting in inhibiting adipogenesis. Taken together, batatasin I might be responsible for the anti-adipogenic effect of DOB via inhibition of PPARγ and C/EBPα and activation of p-AMPK and CPT-1.
収録刊行物
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- Biological & Pharmaceutical Bulletin
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Biological & Pharmaceutical Bulletin 37 (10), 1683-1688, 2014
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390001204633025536
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- NII論文ID
- 130004693793
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- NII書誌ID
- AA10885497
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- COI
- 1:STN:280:DC%2BC2M7ms1aitg%3D%3D
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- ISSN
- 13475215
- 09186158
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- NDL書誌ID
- 025839007
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- PubMed
- 25273391
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可